Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4443000..4443497 Replicon chromosome
Accession NZ_CP110117
Organism Escherichia coli strain 2-331

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag OMD12_RS21790 Protein ID WP_001173343.1
Coordinates 4443000..4443173 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag OMD12_RS21795 Protein ID WP_000398619.1
Coordinates 4443201..4443497 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OMD12_RS21780 (4439670) 4439670..4441127 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
OMD12_RS21785 (4441364) 4441364..4442881 + 1518 WP_001295074.1 lysine--tRNA ligase -
OMD12_RS21790 (4443000) 4443000..4443173 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
OMD12_RS21795 (4443201) 4443201..4443497 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
OMD12_RS21800 (4443724) 4443724..4443996 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
OMD12_RS21805 (4444008) 4444008..4444238 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
OMD12_RS21810 (4444419) 4444419..4446050 + 1632 WP_001216477.1 sensor histidine kinase -
OMD12_RS21815 (4446047) 4446047..4446766 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T263146 WP_001173343.1 NZ_CP110117:c4443173-4443000 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT263146 WP_000398619.1 NZ_CP110117:c4443497-4443201 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References