Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4225395..4225971 Replicon chromosome
Accession NZ_CP110117
Organism Escherichia coli strain 2-331

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag OMD12_RS20640 Protein ID WP_001297643.1
Coordinates 4225684..4225971 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag OMD12_RS20635 Protein ID WP_000063160.1
Coordinates 4225395..4225697 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OMD12_RS20620 (4221991) 4221991..4224141 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
OMD12_RS20625 (4224191) 4224191..4224394 + 204 WP_000467859.1 YbdD/YjiX family protein -
OMD12_RS20630 (4224405) 4224405..4225361 + 957 WP_001297640.1 GTPase -
OMD12_RS20635 (4225395) 4225395..4225697 - 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
OMD12_RS20640 (4225684) 4225684..4225971 - 288 WP_001297643.1 BrnT family toxin Toxin
OMD12_RS20645 (4226170) 4226170..4226866 - 697 Protein_4041 IS1 family transposase -
OMD12_RS20650 (4227181) 4227181..4228413 + 1233 WP_001137030.1 multidrug efflux MFS transporter MdtM -
OMD12_RS20655 (4228454) 4228454..4229734 + 1281 WP_001037390.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4226170..4226379 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T263144 WP_001297643.1 NZ_CP110117:c4225971-4225684 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT263144 WP_000063160.1 NZ_CP110117:c4225697-4225395 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References