Detailed information of TA system TA026309

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 489508..490111
• Accession: NC_017167
• Organism: Alicyclobacillus acidocaldarius subsp. acidocaldarius Tc-4-1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: F8ICH1
• Locus tag: TC41_RS02250
• Protein ID: WP_008336350.1
• Coordinates: 489761..490111 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: TC41_RS02245
• Protein ID: WP_012809791.1
• Coordinates: 489508..489756 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TC41_RS02220	484575..484922	-	348	WP_041694917.1	phage holin family protein	-
TC41_RS02225	485080..485475	+	396	WP_041694918.1	holo-ACP synthase	-
TC41_RS02230	485556..487058	+	1503	WP_014463353.1	bifunctional ADP-dependent NAD(P)H-hydrate dehydratase/NAD(P)H-hydrate epimerase	-
TC41_RS02235	487068..488099	+	1032	WP_014463354.1	outer membrane lipoprotein carrier protein LolA	-
TC41_RS02240	488193..489395	+	1203	WP_014463355.1	alanine racemase	-
TC41_RS02245	489508..489756	+	249	WP_012809791.1	hypothetical protein	Antitoxin
TC41_RS02250	489761..490111	+	351	WP_008336350.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
TC41_RS02255	490200..491453	+	1254	WP_041695642.1	adenosylhomocysteinase	-
TC41_RS02260	491477..492238	+	762	WP_041694919.1	gamma-glutamyl-gamma-aminobutyrate hydrolase family protein	-
TC41_RS02265	492178..493734	-	1557	WP_014463358.1	HD domain-containing protein	-
TC41_RS02270	493959..494273	+	315	WP_014463359.1	DUF1659 domain-containing protein	-
TC41_RS02275	494333..494551	+	219	WP_012809796.1	DUF2922 domain-containing protein	-
TC41_RS02280	494597..494863	-	267	WP_041694920.1	YuzB family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12898.95 Da
• Isoelectric Point: 5.7235

>T26309 WP_008336350.1 NC_017167:489761-490111 [Alicyclobacillus acidocaldarius subsp. acidocaldarius Tc-4-1]
MNVRRGDIFFADLSPVVGSEQGGFRPVLVVQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIKAEKYGLERDSVILLEQL
RTIDKQRLTDKITHLDDEAMKLVNEGLLISLGLVDF

Nucleotide

• Length: 351 bp

>T26309 NC_017167:489761-490111 [Alicyclobacillus acidocaldarius subsp. acidocaldarius Tc-4-1]
TTGAATGTCAGGCGTGGGGACATCTTCTTTGCGGATCTGTCGCCTGTGGTCGGCTCGGAGCAAGGAGGATTTCGCCCCGT
ACTCGTGGTGCAAAATGACATCGGCAACCGCTTCAGCCCGACGGTGATTGTGGCGGCCATCACCGCGCAGATCCAGAAGG
CCAAGTTGCCGACGCACGTGGAGATCAAGGCGGAGAAGTACGGTCTGGAGCGCGATTCCGTGATTCTGCTCGAGCAGCTG
CGCACCATCGATAAGCAGCGCCTGACCGACAAGATCACGCATCTGGACGACGAAGCCATGAAGCTCGTCAACGAGGGGCT
GTTGATCAGCCTCGGCCTGGTGGATTTCTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9304.50 Da
• Isoelectric Point: 7.9798

>AT26309 WP_012809791.1 NC_017167:489508-489756 [Alicyclobacillus acidocaldarius subsp. acidocaldarius Tc-4-1]
MSSSKFNAWNQAAGRESGDKRARDIVREALRMYVSRREPGDLRETMQRGYQEMAPLNLRIACEAFLVEQEAGNTVERLVS
GV

Nucleotide

• Length: 249 bp

>AT26309 NC_017167:489508-489756 [Alicyclobacillus acidocaldarius subsp. acidocaldarius Tc-4-1]
TTGTCGAGTTCGAAATTCAACGCCTGGAATCAAGCTGCGGGTCGCGAATCCGGAGACAAACGCGCGCGTGACATCGTGCG
CGAGGCGCTGCGTATGTATGTATCGAGGAGAGAGCCCGGAGATCTGCGCGAGACCATGCAGCGCGGGTATCAGGAGATGG
CGCCTCTCAACCTGCGCATCGCCTGTGAGGCGTTTCTGGTCGAGCAGGAGGCCGGCAACACCGTGGAGCGCTTGGTGAGC
GGGGTGTGA

Structures

Toxin

• AlphaFold DB: F8ICH1