Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 2782979..2783550 Replicon chromosome
Accession NZ_CP110074
Organism Enterococcus faecalis strain BE5

Toxin (Protein)


Gene name mazF Uniprot ID R3GRA7
Locus tag OLM06_RS13750 Protein ID WP_002360937.1
Coordinates 2782979..2783320 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S4CGQ1
Locus tag OLM06_RS13755 Protein ID WP_002367500.1
Coordinates 2783320..2783550 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OLM06_RS13745 (2778994) 2778994..2782608 - 3615 WP_002389470.1 DNA-directed RNA polymerase subunit beta -
OLM06_RS13750 (2782979) 2782979..2783320 - 342 WP_002360937.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
OLM06_RS13755 (2783320) 2783320..2783550 - 231 WP_002367500.1 hypothetical protein Antitoxin
OLM06_RS13760 (2783605) 2783605..2783784 - 180 WP_111997057.1 hypothetical protein -
OLM06_RS13765 (2783964) 2783964..2784179 - 216 WP_002354768.1 zinc ribbon domain-containing protein -
OLM06_RS13770 (2784318) 2784318..2785310 + 993 WP_002389493.1 biotin--[acetyl-CoA-carboxylase] ligase -
OLM06_RS13775 (2785574) 2785574..2786212 - 639 WP_002378863.1 lytic polysaccharide monooxygenase -
OLM06_RS13780 (2786898) 2786898..2788514 + 1617 WP_002389442.1 phosphatase PAP2/LCP family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13165.41 Da        Isoelectric Point: 9.3988

>T263069 WP_002360937.1 NZ_CP110074:c2783320-2782979 [Enterococcus faecalis]
MNVEKKYIPKKGDIVWIDFDPAAGKEIQKRRPGLVVSRYEFNRKTMFAVICPITSTIKNIPTRYTLPDDIETHGQVVISQ
LKSLDFTERKLSQIEHLPLKDMAKIDQIIEYIF

Download         Length: 342 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8704.69 Da        Isoelectric Point: 4.3313

>AT263069 WP_002367500.1 NZ_CP110074:c2783550-2783320 [Enterococcus faecalis]
MLTTKSRRQGNSVVLTLPTDNGKKPKVDQEYIVMYSDDGTITLVPKIQDPFSEGPEGEYYEKDEWHDLAPEGRELF

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M2A7G9

Antitoxin

Source ID Structure
AlphaFold DB S4CGQ1

References