Detailed information of TA system TA026283

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF(toxin)
• Location: 4793759..4794395
• Replicon: chromosome
• Accession: NC_017138
• Organism: Bacillus megaterium WSH-002

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: D5DWT4
• Locus tag: BMWSH_RS24100
• Protein ID: WP_013055004.1
• Coordinates: 4793759..4794109 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: D5DWT3
• Locus tag: BMWSH_RS24105
• Protein ID: WP_013055003.1
• Coordinates: 4794114..4794395 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BMWSH_RS24065	4789343..4790137	-	795	WP_013055011.1	RNA polymerase sigma factor SigB	-
BMWSH_RS24070	4790103..4790588	-	486	WP_014461967.1	anti-sigma B factor RsbW	-
BMWSH_RS24075	4790585..4790917	-	333	WP_014461968.1	anti-sigma factor antagonist	-
BMWSH_RS24080	4790977..4791987	-	1011	WP_013055008.1	PP2C family protein-serine/threonine phosphatase	-
BMWSH_RS24085	4792001..4792402	-	402	WP_013055007.1	anti-sigma regulatory factor	-
BMWSH_RS24090	4792406..4792762	-	357	WP_013055006.1	STAS domain-containing protein	-
BMWSH_RS24095	4792765..4793601	-	837	WP_014461969.1	STAS domain-containing protein	-
BMWSH_RS24100	4793759..4794109	-	351	WP_013055004.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
BMWSH_RS24105	4794114..4794395	-	282	WP_013055003.1	hypothetical protein	Antitoxin
BMWSH_RS24110	4794596..4795786	-	1191	WP_014461971.1	alanine racemase	-
BMWSH_RS24115	4795900..4796958	-	1059	WP_014461972.1	outer membrane lipoprotein carrier protein LolA	-
BMWSH_RS24120	4797017..4797382	-	366	WP_042992604.1	holo-ACP synthase	-
BMWSH_RS24125	4797447..4798049	+	603	WP_014461974.1	rhomboid family intramembrane serine protease	-
BMWSH_RS24130	4798058..4799029	-	972	WP_042992385.1	UV DNA damage repair endonuclease UvsE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12991.96 Da
• Isoelectric Point: 4.8781

>T26283 WP_013055004.1 NC_017138:c4794109-4793759 [Bacillus megaterium WSH-002]
MVVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDRVDEALQISVGLIDF

Nucleotide

• Length: 351 bp

>T26283 NC_017138:c4794109-4793759 [Bacillus megaterium WSH-002]
TTGGTAGTCAAACGTGGCGACGTTTATTTTGCTGACCTATCTCCTGTTGTTGGCTCAGAACAAGGAGGCGTTCGCCCTGT
ACTTGTCATTCAAAATGATATAGGCAATCGTTTTAGCCCAACTGTTATTGTAGCTGCGATCACTGCTCAAATTCAAAAGG
CAAAGTTACCTACGCATGTTGAAATTGATGCAAAGCGCTATGGATTTGAGCGAGATTCAGTTATTTTACTAGAACAAATT
CGTACTATTGATAAACAGAGGCTAACAGACAAGATTACCCATTTAGATGATGAAATGATGGACAGAGTGGATGAAGCCTT
GCAAATCAGTGTAGGACTTATCGACTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10691.22 Da
• Isoelectric Point: 8.5339

>AT26283 WP_013055003.1 NC_017138:c4794395-4794114 [Bacillus megaterium WSH-002]
VSEASATTEILVRLPKNLVSELDLMVKQENGNRSDFIHQATKLYLRERKKRHIRESMRRGYMEMAKINLNIASEAFLAEY
EADHTVDRLVSGG

Nucleotide

• Length: 282 bp

>AT26283 NC_017138:c4794395-4794114 [Bacillus megaterium WSH-002]
GTGTCTGAAGCGAGCGCAACTACAGAAATCCTAGTAAGATTACCGAAAAATTTAGTATCGGAGCTCGATCTTATGGTTAA
ACAAGAAAATGGCAACCGAAGTGATTTTATTCACCAAGCAACAAAATTGTACCTTCGTGAGCGAAAAAAACGCCATATTC
GCGAATCAATGAGACGTGGATATATGGAGATGGCAAAGATAAACTTAAATATTGCTTCCGAGGCTTTTCTAGCAGAGTAT
GAAGCTGATCATACTGTGGATCGTTTAGTAAGCGGGGGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0B6AMB9