262752

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) rnlAB/RnlA-RnlB
Location 2857054..2858491 Replicon chromosome
Accession NZ_CP110018
Organism Escherichia coli strain DH10B

Toxin (Protein)


Gene name rnlA Uniprot ID P52129
Locus tag OLR78_RS14090 Protein ID WP_000155570.1
Coordinates 2857054..2858127 (+) Length 358 a.a.

Antitoxin (Protein)


Gene name rnlB Uniprot ID P52130
Locus tag OLR78_RS14095 Protein ID WP_000461704.1
Coordinates 2858120..2858491 (+) Length 124 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OLR78_RS14075 (2852487) 2852487..2854676 - 2190 WP_000136402.1 DEAD/DEAH box helicase -
OLR78_RS14080 (2854673) 2854673..2856289 - 1617 WP_000445380.1 anti-bacteriophage protein AbpA -
OLR78_RS14085 (2856649) 2856649..2856912 - 264 WP_000502050.1 DUF1524 domain-containing protein -
OLR78_RS14090 (2857054) 2857054..2858127 + 1074 WP_000155570.1 type II toxin-antitoxin system toxin mRNA endoribonuclease RnlA Toxin
OLR78_RS14095 (2858120) 2858120..2858491 + 372 WP_000461704.1 type II toxin-antitoxin system antitoxin RnlB Antitoxin
OLR78_RS14100 (2858846) 2858846..2859709 + 864 WP_000787597.1 GTPase family protein -
OLR78_RS14105 (2859801) 2859801..2860622 + 822 WP_000197391.1 DUF932 domain-containing protein -
OLR78_RS14110 (2860839) 2860839..2861540 + 702 WP_000189409.1 WYL domain-containing protein -
OLR78_RS14115 (2861581) 2861581..2861817 + 237 WP_001144029.1 protein YpjK -
OLR78_RS14120 (2861817) 2861817..2862260 + 444 WP_000824222.1 lipoprotein YfjS -
OLR78_RS14125 (2862284) 2862284..2862751 + 468 WP_000702495.1 protein YfjT -
OLR78_RS14130 (2863138) 2863138..2863290 - 153 Protein_2763 arsenate reductase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.



Sequences


Toxin        


Download         Length: 358 a.a.        Molecular weight: 40061.13 Da        Isoelectric Point: 6.8293

>T262752 WP_000155570.1 NZ_CP110018:2857054-2858127 [Escherichia coli]
MTIRSYKNLNLVRANIETESRQFIENKNYSIQSIGPMPGSRAGLRVVFTRPGVNLATVDIFYNGDGSTTIQYLTGANRSL
GQELADHLFETINPAEFEQVNMVLQGFVETSVLPVLELSADESHIEFREHSRNAHTVVWKIISTSYQDELTVSLHITTGK
LQIQGRPLSCYRVFTFNLAALLDLQGLEKVLIRQEDGKANIVQQEVARTYLQTVMADAYPHLHVTAEKLLVSGLCVKLAA
PDLPDYCMLLYPELRTIEGVLKSKMSGLGMPVQQPAGFGTYFDKPAAHYILKPQFAATLRPEQINIISTAYTFFNVERHS
LFHMETVVDASRMISDMARLMGKATRAWGIIKDLYIV

Download         Length: 1074 bp


Antitoxin


Download         Length: 124 a.a.        Molecular weight: 13664.69 Da        Isoelectric Point: 4.2391

>AT262752 WP_000461704.1 NZ_CP110018:2858120-2858491 [Escherichia coli]
MFEITGINVSGALKAVVMATGFENPLSSVNEIETKLSALLGSETTGEILFDLLCANGPEWNRFVTLEMKYGRIMLDTAKI
IDEQDVPTHILSKLTFTLRNHPEYLEASVLSPDDVRQVLSMDF

Download         Length: 372 bp


Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 6Y2R
PDB 7AEX
PDB 6Y2Q
PDB 4I8O
PDB 6Y2P
PDB 6Y2S


Antitoxin

Source ID Structure
AlphaFold DB A0A373F4G4

References