Detailed information of TA system TA026274

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 134863..135413
• Replicon: plasmid pAPA32-012
• Accession: NC_017134
• Organism: Acetobacter pasteurianus IFO 3283-32

Toxin (Protein)

• Gene name: relE
• Uniprot ID: C7JII7
• Locus tag: APA32_RS14270
• Protein ID: WP_003618711.1
• Coordinates: 135120..135413 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: D5QDQ9
• Locus tag: APA32_RS14265
• Protein ID: WP_003618686.1
• Coordinates: 134863..135123 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
APA32_RS14235	131130..131711	+	582	WP_012813194.1	group III truncated hemoglobin	-
APA32_RS14240	131739..132038	+	300	WP_012813195.1	DUF1971 domain-containing protein	-
APA32_RS14245	132177..133010	-	834	Protein_121	IS5 family transposase	-
APA32_RS14260	134287..134649	+	363	WP_012813198.1	hypothetical protein	-
APA32_RS14265	134863..135123	+	261	WP_003618686.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
APA32_RS14270	135120..135413	+	294	WP_003618711.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
APA32_RS14275	135615..136598	-	984	WP_012813200.1	Abi family protein	-
APA32_RS14280	136856..138655	-	1800	WP_012813201.1	oleate hydratase	-
APA32_RS14285	138701..139288	-	588	Protein_128	DUF1275 domain-containing protein	-
APA32_RS14290	139274..139519	+	246	Protein_129	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..191443	191443

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Antitoxin

• Toxin YoeB (5-84)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 12579.08 Da
• Isoelectric Point: 12.7867

>T26274 WP_003618711.1 NC_017134:135120-135413 [Acetobacter pasteurianus IFO 3283-32]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK
KKKKKKKKKKKKKKKKKK

Nucleotide

• Length: 294 bp

>T26274 NC_017134:135120-135413 [Acetobacter pasteurianus IFO 3283-32]
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 11169.16 Da
• Isoelectric Point: 12.7344

>AT26274 WP_003618686.1 NC_017134:134863-135123 [Acetobacter pasteurianus IFO 3283-32]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK
KKKKKKK

Nucleotide

• Length: 261 bp

>AT26274 NC_017134:134863-135123 [Acetobacter pasteurianus IFO 3283-32]
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAA

Structures

Antitoxin

• AlphaFold DB: A0A6N3T9M6