Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4453078..4453575 Replicon chromosome
Accession NZ_CP110016
Organism Escherichia coli strain HE_100

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag OLR74_RS21775 Protein ID WP_001173343.1
Coordinates 4453402..4453575 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag OLR74_RS21770 Protein ID WP_000398619.1
Coordinates 4453078..4453374 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OLR74_RS21750 (4449809) 4449809..4450528 - 720 WP_000611288.1 two-component system response regulator DcuR -
OLR74_RS21755 (4450525) 4450525..4452156 - 1632 WP_001216477.1 sensor histidine kinase -
OLR74_RS21760 (4452337) 4452337..4452567 + 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
OLR74_RS21765 (4452579) 4452579..4452851 + 273 WP_000405651.1 GNAT family N-acetyltransferase -
OLR74_RS21770 (4453078) 4453078..4453374 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
OLR74_RS21775 (4453402) 4453402..4453575 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
OLR74_RS21780 (4453694) 4453694..4455211 - 1518 WP_001295074.1 lysine--tRNA ligase -
OLR74_RS21785 (4455448) 4455448..4456905 - 1458 WP_282983371.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T262707 WP_001173343.1 NZ_CP110016:4453402-4453575 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT262707 WP_000398619.1 NZ_CP110016:4453078-4453374 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References