Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 7394..7919 Replicon plasmid pNDM-1-KP424
Accession NZ_CP109986
Organism Klebsiella pneumoniae strain KP424

Toxin (Protein)


Gene name ccdB Uniprot ID W8EAA6
Locus tag OJ961_RS28570 Protein ID WP_008322213.1
Coordinates 7394..7699 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A6C0NE25
Locus tag OJ961_RS28575 Protein ID WP_032934863.1
Coordinates 7701..7919 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OJ961_RS28540 (OJ961_28545) 2531..2647 + 117 Protein_2 IS5 family transposase -
OJ961_RS28545 (OJ961_28550) 2719..3839 + 1121 WP_089046468.1 IS3-like element ISSen4 family transposase -
OJ961_RS28550 (OJ961_28555) 3868..4614 + 747 Protein_4 IS5-like element IS5 family transposase -
OJ961_RS28555 (OJ961_28560) 4776..6098 + 1323 WP_016479957.1 GntP family transporter -
OJ961_RS28560 (OJ961_28565) 6297..7088 - 792 WP_008322208.1 site-specific integrase -
OJ961_RS28565 (OJ961_28570) 7078..7392 - 315 WP_008322211.1 hypothetical protein -
OJ961_RS28570 (OJ961_28575) 7394..7699 - 306 WP_008322213.1 type II toxin-antitoxin system toxin CcdB Toxin
OJ961_RS28575 (OJ961_28580) 7701..7919 - 219 WP_032934863.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
OJ961_RS28590 (OJ961_28595) 10367..11335 - 969 WP_000654811.1 IS5 family transposase -
OJ961_RS28595 (OJ961_28600) 11396..12028 - 633 WP_016479950.1 recombinase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaNDM-1 htpB 1..86272 86272
- inside IScluster/Tn - - 2531..11290 8759


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11450.23 Da        Isoelectric Point: 5.8211

>T262624 WP_008322213.1 NZ_CP109986:c7699-7394 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLVSARLLSDKVSRELYPVVSVGGDPYRLMTTDMASVPATVIGEEV
ADLSHLENDIQNAINLMFRGI

Download         Length: 306 bp

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8297.11 Da        Isoelectric Point: 4.4013

>AT262624 WP_032934863.1 NZ_CP109986:c7919-7701 [Klebsiella pneumoniae]
VKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQEENREGMAEVASFIEANGSFADENRDW

Download         Length: 219 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6C0NE27

Antitoxin

Source ID Structure
AlphaFold DB A0A6C0NE25

References