Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4785026..4785836 Replicon chromosome
Accession NZ_CP109983
Organism Klebsiella pneumoniae strain KP424

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag OJ961_RS23195 Protein ID WP_004178461.1
Coordinates 4785026..4785559 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag OJ961_RS23200 Protein ID WP_002887278.1
Coordinates 4785570..4785836 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OJ961_RS23190 (4783857) 4783857..4784978 + 1122 WP_002887282.1 cupin domain-containing protein -
OJ961_RS23195 (4785026) 4785026..4785559 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
OJ961_RS23200 (4785570) 4785570..4785836 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
OJ961_RS23205 (4785939) 4785939..4787372 - 1434 WP_004178459.1 Cu(+)/Ag(+) sensor histidine kinase -
OJ961_RS23210 (4787362) 4787362..4788045 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
OJ961_RS23215 (4788217) 4788217..4789602 + 1386 WP_004178457.1 efflux transporter outer membrane subunit -
OJ961_RS23220 (4789620) 4789620..4789964 + 345 WP_004197273.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T262613 WP_004178461.1 NZ_CP109983:c4785559-4785026 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT262613 WP_002887278.1 NZ_CP109983:c4785836-4785570 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References