Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5098525..5099150 Replicon chromosome
Accession NZ_CP109963
Organism Klebsiella pneumoniae isolate NY13307

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag OJ970_RS25055 Protein ID WP_002882817.1
Coordinates 5098525..5098908 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag OJ970_RS25060 Protein ID WP_004150355.1
Coordinates 5098908..5099150 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OJ970_RS25040 (OJ970_25045) 5095891..5096793 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
OJ970_RS25045 (OJ970_25050) 5096790..5097425 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
OJ970_RS25050 (OJ970_25055) 5097422..5098351 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
OJ970_RS25055 (OJ970_25060) 5098525..5098908 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
OJ970_RS25060 (OJ970_25065) 5098908..5099150 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
OJ970_RS25065 (OJ970_25070) 5099355..5100272 + 918 WP_002882812.1 alpha/beta hydrolase -
OJ970_RS25070 (OJ970_25075) 5100286..5101227 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
OJ970_RS25075 (OJ970_25080) 5101272..5101709 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
OJ970_RS25080 (OJ970_25085) 5101706..5102566 - 861 WP_002882807.1 virulence factor BrkB family protein -
OJ970_RS25085 (OJ970_25090) 5102560..5103159 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T262564 WP_002882817.1 NZ_CP109963:c5098908-5098525 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT262564 WP_004150355.1 NZ_CP109963:c5099150-5098908 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References