Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 194713..195238 Replicon plasmid p3
Accession NZ_CP109956
Organism Escherichia coli strain NC22

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag OI124_RS26080 Protein ID WP_001159871.1
Coordinates 194933..195238 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag OI124_RS26075 Protein ID WP_000813630.1
Coordinates 194713..194931 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OI124_RS26065 (190939) 190939..191415 - 477 Protein_199 IS256 family transposase -
OI124_RS26070 (191472) 191472..194153 - 2682 WP_000678689.1 hypothetical protein -
OI124_RS26075 (194713) 194713..194931 + 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
OI124_RS26080 (194933) 194933..195238 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
OI124_RS26085 (195239) 195239..196045 + 807 WP_000016968.1 site-specific integrase -
OI124_RS26090 (196725) 196725..197456 - 732 WP_000504262.1 replication initiation protein -
OI124_RS26095 (198077) 198077..199282 + 1206 WP_001442122.1 AAA family ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD vat / iroN / iroE / iroD / iroC / iroB / iutA / iucD / iucC / iucB / iucA 1..255823 255823


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T262546 WP_001159871.1 NZ_CP109956:194933-195238 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT262546 WP_000813630.1 NZ_CP109956:194713-194931 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References