Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 185507..186054 Replicon plasmid p3
Accession NZ_CP109956
Organism Escherichia coli strain NC22

Toxin (Protein)


Gene name parE Uniprot ID G7QEJ9
Locus tag OI124_RS26035 Protein ID WP_001384452.1
Coordinates 185776..186054 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID W0FTF6
Locus tag OI124_RS26030 Protein ID WP_000079942.1
Coordinates 185507..185776 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OI124_RS26010 (181149) 181149..181682 - 534 WP_001372168.1 transcription termination/antitermination NusG family protein -
OI124_RS26015 (182124) 182124..182411 - 288 WP_000074849.1 conjugal transfer protein TraA -
OI124_RS26020 (182329) 182329..182559 - 231 WP_228772074.1 ash family protein -
OI124_RS26025 (183565) 183565..184596 + 1032 WP_000907870.1 plasmid replication initiator RepA -
OI124_RS26030 (185507) 185507..185776 + 270 WP_000079942.1 type II toxin-antitoxin system antitoxin YacA Antitoxin
OI124_RS26035 (185776) 185776..186054 + 279 WP_001384452.1 type II toxin-antitoxin system toxin YacB Toxin
OI124_RS26040 (186100) 186100..186312 + 213 Protein_194 3'-5' exonuclease -
OI124_RS26045 (186571) 186571..187500 - 930 WP_000102288.1 hypothetical protein -
OI124_RS26050 (187937) 187937..189124 - 1188 WP_001697863.1 IS91 family transposase -
OI124_RS26055 (189124) 189124..189489 - 366 WP_000124134.1 hypothetical protein -
OI124_RS26060 (189637) 189637..190929 - 1293 Protein_198 ISL3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD vat / iroN / iroE / iroD / iroC / iroB / iutA / iucD / iucC / iucB / iucA 1..255823 255823


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10762.38 Da        Isoelectric Point: 8.4615

>T262545 WP_001384452.1 NZ_CP109956:185776-186054 [Escherichia coli]
MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVLHTAQKWP

Download         Length: 279 bp

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10282.61 Da        Isoelectric Point: 7.2057

>AT262545 WP_000079942.1 NZ_CP109956:185507-185776 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSATAWRD
EMSRKVAGK

Download         Length: 270 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CL36

Antitoxin

Source ID Structure
AlphaFold DB A0A140JYS5

References