Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 111146..112769 Replicon plasmid p2
Accession NZ_CP109955
Organism Escherichia coli strain NC22

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag OI124_RS24370 Protein ID WP_000088046.1
Coordinates 111444..112769 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag OI124_RS24365 Protein ID WP_024136327.1
Coordinates 111146..111439 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OI124_RS24335 (OI124_24255) 107018..108259 + 1242 WP_001053338.1 VWA domain-containing protein -
OI124_RS24340 (OI124_24260) 108350..108481 + 132 WP_000374059.1 hypothetical protein -
OI124_RS24345 (OI124_24265) 108822..109103 - 282 WP_000077926.1 hypothetical protein -
OI124_RS24350 (OI124_24270) 109153..109344 - 192 WP_000398480.1 hypothetical protein -
OI124_RS24355 (OI124_24275) 109436..109777 - 342 WP_001151575.1 hypothetical protein -
OI124_RS24360 (OI124_24280) 110150..110542 + 393 WP_000341066.1 hypothetical protein -
OI124_RS24365 (OI124_24285) 111146..111439 + 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
OI124_RS24370 (OI124_24290) 111444..112769 + 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
OI124_RS24375 (OI124_24295) 112830..113036 + 207 WP_000134171.1 hypothetical protein -
OI124_RS24380 (OI124_24300) 113138..113548 + 411 WP_000985911.1 hypothetical protein -
OI124_RS24385 (OI124_24305) 113561..114376 + 816 WP_053276223.1 HNH endonuclease signature motif containing protein -
OI124_RS24390 (OI124_24310) 114788..115429 + 642 WP_001567361.1 recombinase family protein -
OI124_RS24395 (OI124_24315) 115793..116773 - 981 WP_021571263.1 helix-turn-helix domain-containing protein -
OI124_RS24400 (OI124_24320) 116898..117677 - 780 WP_021571264.1 MBL fold metallo-hydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) / oqxA / oqxB / ARR-3 / dfrA27 / aadA16 / qacE / sul1 / mph(A) / aph(3')-Ia / aac(3)-IIa / blaCTX-M-64 - 1..234214 234214
- flank IS/Tn - - 114788..115429 641


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T262537 WP_000088046.1 NZ_CP109955:111444-112769 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT262537 WP_024136327.1 NZ_CP109955:111146-111439 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References