Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4577750..4578404 Replicon chromosome
Accession NZ_CP109953
Organism Escherichia coli strain NC22

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag OI124_RS22300 Protein ID WP_000244777.1
Coordinates 4577997..4578404 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag OI124_RS22295 Protein ID WP_000354046.1
Coordinates 4577750..4578016 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OI124_RS22270 (4573038) 4573038..4573781 + 744 WP_000951948.1 SDR family oxidoreductase -
OI124_RS22275 (4573838) 4573838..4575271 - 1434 Protein_4340 6-phospho-beta-glucosidase BglA -
OI124_RS22280 (4575316) 4575316..4575627 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
OI124_RS22285 (4575791) 4575791..4576450 + 660 WP_000250274.1 hemolysin III family protein -
OI124_RS22290 (4576527) 4576527..4577507 - 981 WP_000886061.1 tRNA-modifying protein YgfZ -
OI124_RS22295 (4577750) 4577750..4578016 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
OI124_RS22300 (4577997) 4577997..4578404 + 408 WP_000244777.1 protein YgfX Toxin
OI124_RS22305 (4578444) 4578444..4578965 - 522 WP_001055874.1 flavodoxin FldB -
OI124_RS22310 (4579077) 4579077..4579973 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
OI124_RS22315 (4579998) 4579998..4580708 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
OI124_RS22320 (4580714) 4580714..4582447 + 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T262534 WP_000244777.1 NZ_CP109953:4577997-4578404 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT262534 WP_000354046.1 NZ_CP109953:4577750-4578016 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References