Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 3908283..3909083 Replicon chromosome
Accession NZ_CP109953
Organism Escherichia coli strain NC22

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag OI124_RS18860 Protein ID WP_000342449.1
Coordinates 3908556..3909083 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag OI124_RS18855 Protein ID WP_001277108.1
Coordinates 3908283..3908549 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OI124_RS18835 (3903940) 3903940..3904608 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
OI124_RS18840 (3904601) 3904601..3905659 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
OI124_RS18845 (3905904) 3905904..3906758 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
OI124_RS18850 (3907030) 3907030..3908133 + 1104 WP_001350438.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
OI124_RS18855 (3908283) 3908283..3908549 + 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
OI124_RS18860 (3908556) 3908556..3909083 + 528 WP_000342449.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
OI124_RS18865 (3909080) 3909080..3909463 - 384 WP_000778777.1 aspartate 1-decarboxylase autocleavage activator PanM -
OI124_RS18870 (3909887) 3909887..3910996 + 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
OI124_RS18875 (3911044) 3911044..3911970 + 927 WP_000003004.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
OI124_RS18880 (3911967) 3911967..3913244 + 1278 WP_000803799.1 branched chain amino acid ABC transporter permease LivM -
OI124_RS18885 (3913241) 3913241..3914008 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T262531 WP_000342449.1 NZ_CP109953:3908556-3909083 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT262531 WP_001277108.1 NZ_CP109953:3908283-3908549 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References