Detailed information of TA system TA026253

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1305587..1306398
• Replicon: chromosome
• Accession: NC_017094
• Organism: Leptospirillum ferrooxidans C2-3

Toxin (Protein)

• Gene name: TacT3
• Uniprot ID: I0INX2
• Locus tag: LFE_RS06635
• Protein ID: WP_014449460.1
• Coordinates: 1305862..1306398 (+)
• Length: 179 a.a.

Antitoxin (Protein)

• Gene name: TacA3
• Uniprot ID: I0INX1
• Locus tag: LFE_RS06630
• Protein ID: WP_014449459.1
• Coordinates: 1305587..1305865 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LFE_RS06615	1302290..1303519	-	1230	WP_148272715.1	efflux RND transporter periplasmic adaptor subunit	-
LFE_RS06620	1303539..1304966	-	1428	WP_014449458.1	TolC family protein	-
LFE_RS06625	1305027..1305509	-	483	WP_148272577.1	hypothetical protein	-
LFE_RS06630	1305587..1305865	+	279	WP_014449459.1	DUF1778 domain-containing protein	Antitoxin
LFE_RS06635	1305862..1306398	+	537	WP_014449460.1	GNAT family N-acetyltransferase	Toxin
LFE_RS13670	1306328..1306636	-	309	WP_081495368.1	DUF5615 family PIN-like protein	-
LFE_RS06640	1306633..1306863	-	231	WP_014449462.1	DUF433 domain-containing protein	-
LFE_RS14120	1306958..1307245	-	288	WP_014449463.1	hypothetical protein	-
LFE_RS13920	1307388..1307687	+	300	WP_014449464.1	hypothetical protein	-
LFE_RS13925	1307803..1308003	+	201	WP_014449466.1	hypothetical protein	-
LFE_RS06660	1308176..1308793	-	618	WP_041774880.1	YqhA family protein	-
LFE_RS06670	1309542..1310150	-	609	WP_014449469.1	hypothetical protein	-
LFE_RS06675	1310295..1311365	+	1071	WP_014449470.1	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (11-88)

Sequences

Toxin

Protein

• Length: 179 a.a.
• Molecular weight: 19236.83 Da
• Isoelectric Point: 8.3056

>T26253 WP_014449460.1 NC_017094:1305862-1306398 [Leptospirillum ferrooxidans C2-3]
MTFPAWHEEPVSKKHDRKSFDCGDAELNDFLHRHARQSHDLGGAKTFLAIDNDDNKTILGFYSLAPGAVLYADTPQTFRR
GLAPHDVPGFRLARIATHVCVHGQGLGGQLLASAARRCLLAAAEAGGVLLIIDTKNDRAAKWYASYGAVPLNNRSLTLVM
SLATFAAELKAADQSRPG

Nucleotide

• Length: 537 bp

>T26253 NC_017094:1305862-1306398 [Leptospirillum ferrooxidans C2-3]
TTGACGTTTCCGGCCTGGCACGAAGAGCCGGTTTCCAAGAAGCATGATCGCAAATCGTTCGATTGCGGCGATGCGGAGCT
AAACGATTTTCTGCATCGTCATGCACGTCAAAGCCATGACCTTGGCGGCGCAAAAACCTTCCTGGCTATCGACAACGACG
ATAACAAAACGATCCTCGGCTTCTACAGCTTGGCGCCGGGCGCGGTCTTGTATGCAGATACGCCGCAGACTTTTCGTCGC
GGCCTTGCACCGCACGATGTTCCGGGGTTCCGTCTTGCGCGCATTGCGACGCATGTTTGCGTGCATGGGCAAGGCCTAGG
CGGGCAGCTTCTCGCGTCGGCCGCACGGCGTTGCCTGCTCGCCGCAGCCGAAGCGGGCGGCGTGCTTCTTATCATCGATA
CAAAGAATGACCGCGCGGCAAAATGGTATGCGTCGTATGGCGCAGTTCCACTGAACAATAGGTCACTTACGCTTGTGATG
TCGCTTGCAACCTTTGCCGCTGAACTGAAAGCGGCCGATCAATCGCGTCCGGGCTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10387.20 Da
• Isoelectric Point: 10.7279

>AT26253 WP_014449459.1 NC_017094:1305587-1305865 [Leptospirillum ferrooxidans C2-3]
MPRVAVNENKRMQLRIQPEQKATLMRAAALRNTTLTDFIFQIAMREAHVVIEEAERIRLSERDSLLVLDLLENPPAPNAK
LRGAIAAMPKPS

Nucleotide

• Length: 279 bp

>AT26253 NC_017094:1305587-1305865 [Leptospirillum ferrooxidans C2-3]
ATGCCCAGAGTAGCCGTTAACGAGAACAAGCGGATGCAGCTTCGCATCCAGCCGGAGCAAAAAGCGACGCTTATGCGGGC
GGCCGCGCTTCGGAACACAACCCTAACGGATTTCATCTTTCAGATCGCCATGCGCGAGGCTCATGTCGTGATCGAGGAAG
CAGAGCGGATTCGACTGTCGGAAAGAGACAGCCTGCTGGTGCTGGATCTGCTGGAAAATCCGCCTGCGCCGAATGCCAAG
TTGCGCGGGGCCATTGCCGCGATGCCAAAACCTAGTTGA

Structures

Toxin

• AlphaFold DB: I0INX2

Antitoxin

• AlphaFold DB: I0INX1