Detailed information of TA system TA026251

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 136220..136800
• Replicon: chromosome
• Accession: NC_017094
• Organism: Leptospirillum ferrooxidans C2-3

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: I0IKS1
• Locus tag: LFE_RS00710
• Protein ID: WP_014448365.1
• Coordinates: 136220..136522 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: I0IKS2
• Locus tag: LFE_RS00715
• Protein ID: WP_014448366.1
• Coordinates: 136519..136800 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LFE_RS00655	131532..131939	+	408	WP_014448354.1	IS3 family transposase	-
LFE_RS00660	131981..132388	-	408	WP_014448355.1	hypothetical protein	-
LFE_RS00665	132479..133315	-	837	WP_014448356.1	hypothetical protein	-
LFE_RS00670	133337..133531	-	195	WP_014448357.1	hypothetical protein	-
LFE_RS00680	133808..134095	-	288	WP_014448359.1	putative addiction module antidote protein	-
LFE_RS00685	134107..134397	-	291	WP_014448360.1	type II toxin-antitoxin system RelE/ParE family toxin	-
LFE_RS00690	134566..134778	-	213	WP_014448361.1	DUF2283 domain-containing protein	-
LFE_RS13490	135028..135321	+	294	WP_081495298.1	tyrosine-type recombinase/integrase	-
LFE_RS00700	135564..135917	+	354	WP_014448363.1	hypothetical protein	-
LFE_RS00710	136220..136522	+	303	WP_014448365.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LFE_RS00715	136519..136800	+	282	WP_014448366.1	putative addiction module antidote protein	Antitoxin
LFE_RS14030	136791..136943	-	153	WP_014448367.1	hypothetical protein	-
LFE_RS14035	136972..137112	-	141	WP_158310225.1	hypothetical protein	-
LFE_RS00720	137405..138343	-	939	WP_014448368.1	AraC family transcriptional regulator	-
LFE_RS00725	138524..139528	+	1005	WP_014448369.1	aldo/keto reductase	-
LFE_RS00730	139563..140621	+	1059	WP_014448370.1	NAD(P)-dependent alcohol dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	128618..148710	20092

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-95)

Antitoxin

• Uncharacterized protein HI1420 (15-92)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11234.01 Da
• Isoelectric Point: 9.6544

>T26251 WP_014448365.1 NC_017094:136220-136522 [Leptospirillum ferrooxidans C2-3]
MIAVQQTETFAHWLSSLKDRSGLVRILSRLDRIAMGNFGDVKPVGEGVSEIRIDTGPGYRLYFVRQENTLILLLCGGDKS
TQDRDISLAKELKKTLEKKP

Nucleotide

• Length: 303 bp

>T26251 NC_017094:136220-136522 [Leptospirillum ferrooxidans C2-3]
ATGATTGCCGTCCAGCAGACAGAAACCTTCGCGCATTGGCTTTCCTCTCTGAAGGATCGATCCGGACTGGTCCGGATCCT
GTCCCGCCTGGACAGAATCGCGATGGGAAATTTTGGTGACGTAAAGCCGGTAGGAGAGGGCGTTTCGGAAATCCGAATCG
ATACGGGACCCGGATACCGCCTGTATTTCGTCCGCCAGGAGAACACGTTGATTCTCCTGCTTTGCGGCGGGGACAAATCG
ACGCAAGACCGCGACATCTCTCTGGCAAAGGAATTGAAGAAAACCCTGGAGAAGAAACCATGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10248.86 Da
• Isoelectric Point: 5.1373

>AT26251 WP_014448366.1 NC_017094:136519-136800 [Leptospirillum ferrooxidans C2-3]
MKEKILEFDPSVYLDSEETIAEYLTVTLEENDPDLLLAALSNVAKARGMSQIARESGLGRESLYKALTPGSKPRFETIMK
VMHALGVKLAVHA

Nucleotide

• Length: 282 bp

>AT26251 NC_017094:136519-136800 [Leptospirillum ferrooxidans C2-3]
ATGAAAGAAAAAATTCTGGAATTCGATCCGTCGGTATATCTCGACAGCGAGGAAACCATTGCGGAATACCTGACCGTCAC
CCTTGAGGAGAACGATCCCGATCTTCTCCTAGCGGCTCTGTCCAACGTGGCCAAAGCACGCGGAATGTCCCAGATAGCTC
GGGAATCGGGGCTTGGGCGGGAAAGTCTTTACAAAGCCCTGACCCCCGGCTCCAAACCGCGCTTCGAAACGATCATGAAG
GTGATGCACGCGCTGGGCGTCAAACTGGCCGTTCACGCCTGA

Structures

Toxin

• AlphaFold DB: I0IKS1

Antitoxin

• AlphaFold DB: I0IKS2