Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4767335..4768145 Replicon chromosome
Accession NZ_CP109948
Organism Klebsiella pneumoniae strain KP1650

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag OIS41_RS23515 Protein ID WP_002887280.1
Coordinates 4767335..4767868 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag OIS41_RS23520 Protein ID WP_002887278.1
Coordinates 4767879..4768145 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OIS41_RS23510 (OIS41_23515) 4766166..4767287 + 1122 WP_002887282.1 cupin domain-containing protein -
OIS41_RS23515 (OIS41_23520) 4767335..4767868 - 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
OIS41_RS23520 (OIS41_23525) 4767879..4768145 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
OIS41_RS23525 (OIS41_23530) 4768248..4769681 - 1434 WP_002887275.1 Cu(+)/Ag(+) sensor histidine kinase -
OIS41_RS23530 (OIS41_23535) 4769671..4770354 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
OIS41_RS23535 (OIS41_23540) 4770526..4771911 + 1386 WP_002887267.1 efflux transporter outer membrane subunit -
OIS41_RS23540 (OIS41_23545) 4771929..4772273 + 345 WP_002887266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T262501 WP_002887280.1 NZ_CP109948:c4767868-4767335 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT262501 WP_002887278.1 NZ_CP109948:c4768145-4767879 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References