Detailed information of TA system TA026250

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 133808..134397
• Replicon: chromosome
• Accession: NC_017094
• Organism: Leptospirillum ferrooxidans C2-3

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: I0IKR6
• Locus tag: LFE_RS00685
• Protein ID: WP_014448360.1
• Coordinates: 134107..134397 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: I0IKR5
• Locus tag: LFE_RS00680
• Protein ID: WP_014448359.1
• Coordinates: 133808..134095 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LFE_RS00640	128960..130333	-	1374	WP_014448351.1	putative DNA binding domain-containing protein	-
LFE_RS00645	130335..131048	-	714	WP_014448352.1	hypothetical protein	-
LFE_RS00650	131172..131357	+	186	WP_014448353.1	helix-turn-helix domain-containing protein	-
LFE_RS00655	131532..131939	+	408	WP_014448354.1	IS3 family transposase	-
LFE_RS00660	131981..132388	-	408	WP_014448355.1	hypothetical protein	-
LFE_RS00665	132479..133315	-	837	WP_014448356.1	hypothetical protein	-
LFE_RS00670	133337..133531	-	195	WP_014448357.1	hypothetical protein	-
LFE_RS00680	133808..134095	-	288	WP_014448359.1	putative addiction module antidote protein	Antitoxin
LFE_RS00685	134107..134397	-	291	WP_014448360.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LFE_RS00690	134566..134778	-	213	WP_014448361.1	DUF2283 domain-containing protein	-
LFE_RS13490	135028..135321	+	294	WP_081495298.1	tyrosine-type recombinase/integrase	-
LFE_RS00700	135564..135917	+	354	WP_014448363.1	hypothetical protein	-
LFE_RS00710	136220..136522	+	303	WP_014448365.1	type II toxin-antitoxin system RelE/ParE family toxin	-
LFE_RS00715	136519..136800	+	282	WP_014448366.1	putative addiction module antidote protein	-
LFE_RS14030	136791..136943	-	153	WP_014448367.1	hypothetical protein	-
LFE_RS14035	136972..137112	-	141	WP_158310225.1	hypothetical protein	-
LFE_RS00720	137405..138343	-	939	WP_014448368.1	AraC family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	128618..148710	20092

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-95)

Antitoxin

• Uncharacterized protein HI1420 (3-93)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 10798.31 Da
• Isoelectric Point: 7.9923

>T26250 WP_014448360.1 NC_017094:c134397-134107 [Leptospirillum ferrooxidans C2-3]
MIEVRQTDTYSEWFSELRDRAAKARIDIRIRRLSLGNPGDVKPVGSGISEMRIDYGPGYRIYFTQCGPALVILLAGGDKS
TQEKDIRTAHELADKL

Nucleotide

• Length: 291 bp

>T26250 NC_017094:c134397-134107 [Leptospirillum ferrooxidans C2-3]
ATGATCGAAGTCCGTCAGACTGATACTTATTCCGAATGGTTTTCAGAACTTCGAGATCGAGCCGCGAAGGCCAGAATTGA
TATTCGGATTCGCCGGCTTTCCCTCGGAAATCCTGGAGACGTGAAACCGGTAGGGTCCGGTATCTCCGAAATGCGAATAG
ACTACGGACCAGGATATCGCATTTATTTCACGCAATGCGGGCCCGCGCTTGTTATTCTTCTGGCCGGGGGAGACAAGAGT
ACACAGGAAAAGGATATCAGAACGGCCCATGAACTTGCCGACAAACTTTAG

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10277.76 Da
• Isoelectric Point: 5.1585

>AT26250 WP_014448359.1 NC_017094:c134095-133808 [Leptospirillum ferrooxidans C2-3]
MRKTKTRLWDPAEHLETEEDMAAYLEAALEDGDPVLVAAALGDIARAKGMTQIARETGLGRESLYKALSAEGNPEFATVL
KVLRSLGLRLHATVG

Nucleotide

• Length: 288 bp

>AT26250 NC_017094:c134095-133808 [Leptospirillum ferrooxidans C2-3]
ATGCGAAAAACAAAAACCCGACTTTGGGATCCGGCAGAACATCTGGAAACCGAAGAAGATATGGCCGCTTATCTTGAAGC
GGCTCTCGAAGATGGAGACCCTGTCCTTGTGGCAGCGGCTCTGGGAGATATAGCCCGGGCCAAGGGGATGACGCAAATTG
CCCGCGAAACCGGCCTTGGCCGTGAAAGCCTTTATAAGGCCCTTTCGGCAGAGGGAAATCCTGAGTTTGCAACGGTTCTC
AAAGTTCTCCGATCCCTTGGGTTACGACTTCATGCCACGGTCGGTTGA

Structures

Toxin

• AlphaFold DB: I0IKR6

Antitoxin

• AlphaFold DB: I0IKR5