Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 2764545..2765196 Replicon chromosome
Accession NZ_CP109945
Organism Lacticaseibacillus paracasei subsp. tolerans strain FX-6-1

Toxin (Protein)


Gene name pemK Uniprot ID K6S8L5
Locus tag OH135_RS13385 Protein ID WP_003567661.1
Coordinates 2764545..2764928 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID K6RKI5
Locus tag OH135_RS13390 Protein ID WP_003567665.1
Coordinates 2764948..2765196 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OH135_RS13380 (OH135_13380) 2763840..2764361 - 522 WP_003567657.1 QueT transporter family protein -
OH135_RS13385 (OH135_13385) 2764545..2764928 - 384 WP_003567661.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
OH135_RS13390 (OH135_13390) 2764948..2765196 - 249 WP_003567665.1 antitoxin Antitoxin
OH135_RS13395 (OH135_13395) 2765264..2766400 - 1137 WP_076652501.1 alanine racemase -
OH135_RS13400 (OH135_13400) 2766387..2766761 - 375 WP_003567669.1 holo-ACP synthase -
OH135_RS13405 (OH135_13405) 2766931..2768439 - 1509 WP_003567670.1 DEAD/DEAH box helicase -
OH135_RS13410 (OH135_13410) 2768738..2770126 - 1389 WP_003591978.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 13773.03 Da        Isoelectric Point: 10.9764

>T262482 WP_003567661.1 NZ_CP109945:c2764928-2764545 [Lacticaseibacillus paracasei subsp. tolerans]
MDVSVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSTKMAAVDRALAISVGLVSLPKPKTYNKN

Download         Length: 384 bp

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8938.08 Da        Isoelectric Point: 4.4610

>AT262482 WP_003567665.1 NZ_CP109945:c2765196-2764948 [Lacticaseibacillus paracasei subsp. tolerans]
MKKQTSVAVTFDDAIKARLDAYCELNDIDAQAVITEAVATFLKSQAPEINQLISGYVAMGQINTEISHAFSACESEAYAH
IR

Download         Length: 249 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K6S8L5

Antitoxin

Source ID Structure
AlphaFold DB A0A0E2BNY2

References