Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 2732396..2733047 Replicon chromosome
Accession NZ_CP109944
Organism Lacticaseibacillus paracasei subsp. tolerans strain FX-6

Toxin (Protein)


Gene name pemK Uniprot ID K6S8L5
Locus tag OH134_RS13370 Protein ID WP_003567661.1
Coordinates 2732396..2732779 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID K6RKI5
Locus tag OH134_RS13375 Protein ID WP_003567665.1
Coordinates 2732799..2733047 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OH134_RS13365 (OH134_13365) 2731691..2732212 - 522 WP_003567657.1 QueT transporter family protein -
OH134_RS13370 (OH134_13370) 2732396..2732779 - 384 WP_003567661.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
OH134_RS13375 (OH134_13375) 2732799..2733047 - 249 WP_003567665.1 antitoxin Antitoxin
OH134_RS13380 (OH134_13380) 2733115..2734251 - 1137 WP_076652501.1 alanine racemase -
OH134_RS13385 (OH134_13385) 2734238..2734612 - 375 WP_003567669.1 holo-ACP synthase -
OH134_RS13390 (OH134_13390) 2734782..2736290 - 1509 WP_003567670.1 DEAD/DEAH box helicase -
OH134_RS13395 (OH134_13395) 2736589..2737977 - 1389 WP_003591978.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 13773.03 Da        Isoelectric Point: 10.9764

>T262480 WP_003567661.1 NZ_CP109944:c2732779-2732396 [Lacticaseibacillus paracasei subsp. tolerans]
MDVSVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSTKMAAVDRALAISVGLVSLPKPKTYNKN

Download         Length: 384 bp

>T262480 NZ_CP109944:c2732779-2732396 [Lacticaseibacillus paracasei subsp. tolerans]
ATGGATGTGTCCGTAAAACGCGGCGATGTTTTTTTCGCCGATCTGTCTCCGGTTGTCGGCTCCGAACAGGGCGGCAACCG
GCCAGTACTTATTATTCAAAACAACGTTGGCAACCATTACAGTCCCACAGTGATTGTGGCAGCCATTACCTCCAAGATTC
AAAAACCCAAGATGCCGACGCATGTCGGGTTACGGGCCAAGCAAGATGGCGTTGAACGCAACTCGGTCATTTTACTTGAG
CAAATTCGCACGATTGACAAGCAACGGCTACAAGCCCGCGTGACAGCTCTGTCAAGCACCAAAATGGCAGCAGTCGACCG
CGCGTTGGCGATTTCAGTGGGTCTGGTCAGTTTGCCCAAGCCCAAAACATATAATAAGAATTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8938.08 Da        Isoelectric Point: 4.4610

>AT262480 WP_003567665.1 NZ_CP109944:c2733047-2732799 [Lacticaseibacillus paracasei subsp. tolerans]
MKKQTSVAVTFDDAIKARLDAYCELNDIDAQAVITEAVATFLKSQAPEINQLISGYVAMGQINTEISHAFSACESEAYAH
IR

Download         Length: 249 bp

>AT262480 NZ_CP109944:c2733047-2732799 [Lacticaseibacillus paracasei subsp. tolerans]
ATGAAAAAGCAAACGTCAGTAGCGGTAACGTTTGATGATGCCATTAAGGCGCGGTTGGATGCCTATTGTGAATTGAATGA
CATTGACGCTCAGGCTGTGATTACGGAGGCAGTGGCGACATTTTTGAAGTCACAGGCTCCCGAAATCAACCAATTGATTT
CTGGCTATGTAGCAATGGGCCAGATCAATACAGAGATCAGTCATGCTTTCTCTGCCTGCGAGAGCGAGGCATACGCCCAT
ATTCGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K6S8L5

Antitoxin

Source ID Structure
AlphaFold DB A0A0E2BNY2

References