262478

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 4285978..4286631 Replicon chromosome
Accession NZ_CP109943
Organism Brevibacillus sp. WF146

Toxin (Protein)


Gene name mazF Uniprot ID J2PLP3
Locus tag A6764_RS21295 Protein ID WP_005830205.1
Coordinates 4285978..4286328 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A553KJJ6
Locus tag A6764_RS21300 Protein ID WP_029101003.1
Coordinates 4286332..4286631 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A6764_RS21270 (A6764_21270) 4282776..4283564 - 789 WP_065068247.1 RNA polymerase sigma factor SigB -
A6764_RS21275 (A6764_21275) 4283533..4284024 - 492 WP_065068248.1 anti-sigma B factor RsbW -
A6764_RS21280 (A6764_21280) 4283997..4284350 - 354 WP_029101006.1 STAS domain-containing protein -
A6764_RS21285 (A6764_21285) 4284377..4285378 - 1002 WP_029101005.1 PP2C family protein-serine/threonine phosphatase -
A6764_RS21290 (A6764_21290) 4285375..4285851 - 477 WP_083989938.1 anti-sigma regulatory factor -
A6764_RS21295 (A6764_21295) 4285978..4286328 - 351 WP_005830205.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
A6764_RS21300 (A6764_21300) 4286332..4286631 - 300 WP_029101003.1 CopG family ribbon-helix-helix protein Antitoxin
A6764_RS21305 (A6764_21305) 4286802..4288007 - 1206 WP_044899694.1 alanine racemase -
A6764_RS21310 (A6764_21310) 4288016..4288456 - 441 WP_035299230.1 hypothetical protein -
A6764_RS21315 (A6764_21315) 4288658..4289692 - 1035 WP_029100999.1 DUF4367 domain-containing protein -
A6764_RS21320 (A6764_21320) 4289931..4290317 - 387 WP_044899968.1 holo-ACP synthase -
A6764_RS21325 (A6764_21325) 4290716..4290880 - 165 WP_065068250.1 gamma-type small acid-soluble spore protein -
A6764_RS21330 (A6764_21330) 4291107..4291358 + 252 WP_029100996.1 glutaredoxin family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(15-52)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12917.86 Da        Isoelectric Point: 4.8435

>T262478 WP_005830205.1 NZ_CP109943:c4286328-4285978 [Brevibacillus sp. WF146]
VIVKRGDVFFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKTYGFDRDSVILLEQI
RTIDKQRLTDKITHLDDEMMDRVNESLQISLGLIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 11335.31 Da        Isoelectric Point: 10.3594

>AT262478 WP_029101003.1 NZ_CP109943:c4286631-4286332 [Brevibacillus sp. WF146]
MVGGGFVLSKSNTKRIMISLPQHLLQEVDGVIQKEKSNRSEFIRQAMHLYLKERKKRVIRETMQKGYMEMAKINLKMASE
AFGAEEEADHTLIRLVSGV

Download         Length: 300 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB J2PLP3

Antitoxin

Source ID Structure
AlphaFold DB A0A553KJJ6

References