Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4652086..4652583 Replicon chromosome
Accession NZ_CP109929
Organism Escherichia coli O6:K2:H1 strain C 691-04A

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag N8A71_RS22300 Protein ID WP_001173343.1
Coordinates 4652086..4652259 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag N8A71_RS22305 Protein ID WP_001312331.1
Coordinates 4652287..4652583 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N8A71_RS22290 (4648756) 4648756..4650213 + 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -
N8A71_RS22295 (4650450) 4650450..4651967 + 1518 WP_000003806.1 lysine--tRNA ligase -
N8A71_RS22300 (4652086) 4652086..4652259 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
N8A71_RS22305 (4652287) 4652287..4652583 - 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
N8A71_RS22310 (4652812) 4652812..4653084 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
N8A71_RS22315 (4653096) 4653096..4653326 - 231 WP_000371715.1 4Fe-4S mono-cluster protein YjdI -
N8A71_RS22320 (4653507) 4653507..4655138 + 1632 WP_001216466.1 sensor histidine kinase -
N8A71_RS22325 (4655135) 4655135..4655854 + 720 WP_000611291.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4621864..4652583 30719


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T262414 WP_001173343.1 NZ_CP109929:c4652259-4652086 [Escherichia coli O6:K2:H1]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT262414 WP_001312331.1 NZ_CP109929:c4652583-4652287 [Escherichia coli O6:K2:H1]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References