Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4289035..4289611 Replicon chromosome
Accession NZ_CP109929
Organism Escherichia coli O6:K2:H1 strain C 691-04A

Toxin (Protein)


Gene name shpA Uniprot ID A0A061KXE4
Locus tag N8A71_RS20560 Protein ID WP_001295743.1
Coordinates 4289324..4289611 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A066STF6
Locus tag N8A71_RS20555 Protein ID WP_000063148.1
Coordinates 4289035..4289337 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N8A71_RS20540 (4285631) 4285631..4287781 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
N8A71_RS20545 (4287831) 4287831..4288034 + 204 WP_000467859.1 YbdD/YjiX family protein -
N8A71_RS20550 (4288045) 4288045..4289001 + 957 WP_001295745.1 GTPase -
N8A71_RS20555 (4289035) 4289035..4289337 - 303 WP_000063148.1 BrnA antitoxin family protein Antitoxin
N8A71_RS20560 (4289324) 4289324..4289611 - 288 WP_001295743.1 BrnT family toxin Toxin
N8A71_RS20565 (4289808) 4289808..4290974 + 1167 WP_000800831.1 restriction endonuclease -
N8A71_RS20570 (4291038) 4291038..4292657 + 1620 WP_001029745.1 class I SAM-dependent DNA methyltransferase -
N8A71_RS20575 (4292647) 4292647..4293951 + 1305 WP_000535012.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11156.62 Da        Isoelectric Point: 7.4697

>T262411 WP_001295743.1 NZ_CP109929:c4289611-4289324 [Escherichia coli O6:K2:H1]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11592.12 Da        Isoelectric Point: 9.4646

>AT262411 WP_000063148.1 NZ_CP109929:c4289337-4289035 [Escherichia coli O6:K2:H1]
MSMVKHKRGTPSTLDVQHEAELKALANKFDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KXE4

Antitoxin

Source ID Structure
AlphaFold DB A0A066STF6

References