Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 2566312..2567900 Replicon chromosome
Accession NZ_CP109929
Organism Escherichia coli O6:K2:H1 strain C 691-04A

Toxin (Protein)


Gene name hipA Uniprot ID A0A1L1KDK2
Locus tag N8A71_RS12250 Protein ID WP_001125431.1
Coordinates 2566578..2567900 (+) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID V0VAH2
Locus tag N8A71_RS12245 Protein ID WP_001304368.1
Coordinates 2566312..2566578 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N8A71_RS12230 (2562680) 2562680..2564131 + 1452 WP_001566699.1 tagaturonate reductase -
N8A71_RS12235 (2564328) 2564328..2565242 + 915 WP_001443133.1 bestrophin family protein -
N8A71_RS12240 (2565246) 2565246..2566004 - 759 WP_001286540.1 trans-aconitate 2-methyltransferase -
N8A71_RS12245 (2566312) 2566312..2566578 + 267 WP_001304368.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
N8A71_RS12250 (2566578) 2566578..2567900 + 1323 WP_001125431.1 type II toxin-antitoxin system serine/threonine protein kinase toxin HipA Toxin
N8A71_RS12255 (2568210) 2568210..2568617 - 408 Protein_2405 acetylxylan esterase -
N8A71_RS12260 (2568620) 2568620..2569201 - 582 Protein_2406 LysR family transcriptional regulator -
N8A71_RS12265 (2569350) 2569350..2570132 + 783 WP_001221834.1 SDR family oxidoreductase -
N8A71_RS12270 (2570376) 2570376..2570483 - 108 Protein_2408 hypothetical protein -
N8A71_RS12275 (2570908) 2570908..2571471 + 564 WP_001327671.1 type 1 fimbrial major subunit FimA -
N8A71_RS12280 (2571833) 2571833..2572543 + 711 WP_264427193.1 fimbria/pilus periplasmic chaperone -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(4-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(14-69)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 49310.02 Da        Isoelectric Point: 8.6972

>T262404 WP_001125431.1 NZ_CP109929:2566578-2567900 [Escherichia coli O6:K2:H1]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVKRFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIAQIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKNSLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Download         Length: 1323 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9957.31 Da        Isoelectric Point: 4.9632

>AT262404 WP_001304368.1 NZ_CP109929:2566312-2566578 [Escherichia coli O6:K2:H1]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTSLTTFFKILQSLELSMTLCDAKNASPEA
AEQQDLEW

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1L1KDK2

Antitoxin

Source ID Structure
AlphaFold DB V0VAH2

References