Detailed information of TA system TA026240

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1253051..1253968
• Accession: NC_017061
• Organism: Bacillus amyloliquefaciens subsp. plantarum YAU B9601-Y2

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: BANAU_RS06480
• Protein ID: WP_007407256.1
• Coordinates: 1253222..1253968 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2C3Z8
• Locus tag: BANAU_RS06475
• Protein ID: WP_014417527.1
• Coordinates: 1253051..1253221 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BANAU_RS06430	1248257..1248769	+	513	WP_012117362.1	sigma-70 family RNA polymerase sigma factor	-
BANAU_RS21615	1248882..1249541	+	660	WP_014417523.1	terminase	-
BANAU_RS06440	1249439..1249906	+	468	Protein_1215	hypothetical protein	-
BANAU_RS06445	1249919..1250290	+	372	WP_014417525.1	hypothetical protein	-
BANAU_RS06450	1250295..1250492	+	198	WP_007610833.1	XkdX family protein	-
BANAU_RS06455	1250549..1251310	+	762	WP_014417526.1	hypothetical protein	-
BANAU_RS06460	1251362..1251625	+	264	WP_003154815.1	hemolysin XhlA family protein	-
BANAU_RS06465	1251639..1251902	+	264	WP_003154813.1	phage holin	-
BANAU_RS06470	1251916..1252794	+	879	WP_007407257.1	N-acetylmuramoyl-L-alanine amidase	-
BANAU_RS06475	1253051..1253221	-	171	WP_014417527.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
BANAU_RS06480	1253222..1253968	-	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
BANAU_RS06485	1254072..1255070	-	999	WP_003154805.1	inorganic phosphate transporter	-
BANAU_RS06490	1255083..1255700	-	618	WP_003154804.1	DUF47 domain-containing protein	-
BANAU_RS06495	1255986..1257302	-	1317	WP_007610842.1	amino acid permease	-
BANAU_RS06500	1257624..1258574	+	951	WP_014417529.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T26240 WP_007407256.1 NC_017061:c1253968-1253222 [Bacillus velezensis YAU B9601-Y2]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T26240 NC_017061:c1253968-1253222 [Bacillus velezensis YAU B9601-Y2]
ATGCTTTTGTTTTTTCAGATTATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTACTGGGCTCCATGGTCTATTGGA
CATATGATCCCGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCCGCTTATGCCGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCAGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAGAACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.78 Da
• Isoelectric Point: 10.9951

>AT26240 WP_014417527.1 NC_017061:c1253221-1253051 [Bacillus velezensis YAU B9601-Y2]
MEQAFQNGQPEKRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT26240 NC_017061:c1253221-1253051 [Bacillus velezensis YAU B9601-Y2]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAAAAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2C3Z8