Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4483221..4483718 Replicon chromosome
Accession NZ_CP109924
Organism Escherichia coli O125ac:K+:H10 strain C 236-04A

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag N8A72_RS21885 Protein ID WP_001173343.1
Coordinates 4483221..4483394 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID J7RC38
Locus tag N8A72_RS21890 Protein ID WP_001425728.1
Coordinates 4483422..4483718 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N8A72_RS21875 (4479891) 4479891..4481348 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
N8A72_RS21880 (4481585) 4481585..4483102 + 1518 WP_001295074.1 lysine--tRNA ligase -
N8A72_RS21885 (4483221) 4483221..4483394 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
N8A72_RS21890 (4483422) 4483422..4483718 - 297 WP_001425728.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
N8A72_RS21895 (4483945) 4483945..4484217 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
N8A72_RS21900 (4484229) 4484229..4484459 - 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
N8A72_RS21905 (4484640) 4484640..4486271 + 1632 WP_001216486.1 sensor histidine kinase -
N8A72_RS21910 (4486268) 4486268..4486987 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4447720..4483718 35998


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T262393 WP_001173343.1 NZ_CP109924:c4483394-4483221 [Escherichia coli O125ac:K+:H10]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11466.99 Da        Isoelectric Point: 4.2544

>AT262393 WP_001425728.1 NZ_CP109924:c4483718-4483422 [Escherichia coli O125ac:K+:H10]
MEGKNKFNTYVVSFNYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB J7RC38

References