Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4228149..4228725 Replicon chromosome
Accession NZ_CP109924
Organism Escherichia coli O125ac:K+:H10 strain C 236-04A

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag N8A72_RS20600 Protein ID WP_001297643.1
Coordinates 4228438..4228725 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag N8A72_RS20595 Protein ID WP_000063160.1
Coordinates 4228149..4228451 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N8A72_RS20580 (4224745) 4224745..4226895 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
N8A72_RS20585 (4226945) 4226945..4227148 + 204 WP_000467859.1 YbdD/YjiX family protein -
N8A72_RS20590 (4227159) 4227159..4228115 + 957 WP_001297640.1 GTPase -
N8A72_RS20595 (4228149) 4228149..4228451 - 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
N8A72_RS20600 (4228438) 4228438..4228725 - 288 WP_001297643.1 BrnT family toxin Toxin
N8A72_RS20605 (4228924) 4228924..4229620 - 697 Protein_4050 IS1 family transposase -
N8A72_RS20610 (4229935) 4229935..4231167 + 1233 WP_001137031.1 multidrug efflux MFS transporter MdtM -
N8A72_RS20615 (4231208) 4231208..4232488 + 1281 WP_001037390.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4228924..4229133 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T262390 WP_001297643.1 NZ_CP109924:c4228725-4228438 [Escherichia coli O125ac:K+:H10]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT262390 WP_000063160.1 NZ_CP109924:c4228451-4228149 [Escherichia coli O125ac:K+:H10]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References