Detailed information of TA system TA026239

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 474555..475192
• Accession: NC_017061
• Organism: Bacillus amyloliquefaciens subsp. plantarum YAU B9601-Y2

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: BANAU_RS02430
• Protein ID: WP_003156187.1
• Coordinates: 474842..475192 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: BANAU_RS02425
• Protein ID: WP_003156188.1
• Coordinates: 474555..474836 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BANAU_RS02405	470920..471519	-	600	WP_003156193.1	rhomboid family intramembrane serine protease	-
BANAU_RS02410	471612..471977	+	366	WP_014304402.1	holo-ACP synthase	-
BANAU_RS02415	472142..473149	+	1008	WP_007410230.1	outer membrane lipoprotein carrier protein LolA	-
BANAU_RS02420	473266..474435	+	1170	WP_014416966.1	alanine racemase	-
BANAU_RS02425	474555..474836	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
BANAU_RS02430	474842..475192	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
BANAU_RS02435	475310..476131	+	822	WP_003156182.1	STAS domain-containing protein	-
BANAU_RS02440	476136..476501	+	366	WP_007609584.1	RsbT antagonist protein RsbS	-
BANAU_RS02445	476504..476905	+	402	WP_003156178.1	anti-sigma regulatory factor	-
BANAU_RS02450	476917..477924	+	1008	WP_007609589.1	PP2C family protein-serine/threonine phosphatase	-
BANAU_RS02455	477988..478317	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
BANAU_RS02460	478314..478796	+	483	WP_007609591.1	anti-sigma B factor RsbW	-
BANAU_RS02465	478762..479550	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
BANAU_RS02470	479550..480152	+	603	WP_007410234.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T26239 WP_003156187.1 NC_017061:474842-475192 [Bacillus velezensis YAU B9601-Y2]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T26239 NC_017061:474842-475192 [Bacillus velezensis YAU B9601-Y2]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT26239 WP_003156188.1 NC_017061:474555-474836 [Bacillus velezensis YAU B9601-Y2]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT26239 NC_017061:474555-474836 [Bacillus velezensis YAU B9601-Y2]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5