Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-couple_hipB
Location 422828..424647 Replicon plasmid PRA1
Accession NC_017060
Organism Rahnella aquatilis HX2

Toxin (Protein)


Gene name hipA Uniprot ID H8P0A1
Locus tag Q7S_RS24450 Protein ID WP_013578107.1
Coordinates 423298..424647 (-) Length 450 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID H8P0A0
Locus tag Q7S_RS24445 Protein ID WP_013578106.1
Coordinates 422828..423301 (-) Length 158 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Q7S_RS24425 418070..419536 + 1467 WP_014416645.1 HAMP domain-containing histidine kinase -
Q7S_RS24430 419783..421282 + 1500 WP_041673472.1 UbiD family decarboxylase -
Q7S_RS24435 421478..422332 + 855 WP_014416647.1 LysR family transcriptional regulator -
Q7S_RS24440 422448..422768 + 321 WP_013578105.1 multidrug efflux SMR transporter -
Q7S_RS24445 422828..423301 - 474 WP_013578106.1 helix-turn-helix domain-containing protein Antitoxin
Q7S_RS24450 423298..424647 - 1350 WP_013578107.1 type II toxin-antitoxin system HipA family toxin Toxin
Q7S_RS24455 424843..425064 - 222 WP_014416648.1 DUF1471 domain-containing protein -
Q7S_RS24460 425232..425855 - 624 WP_014416649.1 NAD(P)H-binding protein -
Q7S_RS24465 425852..426373 - 522 WP_041673402.1 heme utilization cystosolic carrier protein HutX -
Q7S_RS24470 426351..427676 - 1326 WP_014416651.1 heme anaerobic degradation radical SAM methyltransferase ChuW/HutW -
Q7S_RS24475 427789..428163 - 375 WP_041673403.1 hypothetical protein -
Q7S_RS24480 428542..428760 + 219 WP_013578112.1 YgdI/YgdR family lipoprotein -
Q7S_RS24485 428925..429218 + 294 Protein_403 iron dicitrate ABC transporter ATP-binding protein -
Q7S_RS26290 429314..429532 + 219 WP_014416654.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaRAHN-2 mgtB / vipA/tssB / vipB/tssC / hcp/tssD / clpV/tssH / hcp/tssD / hcp/tssD 1..570951 570951


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(166-381)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-139)


Sequences

Toxin        


Download         Length: 450 a.a.        Molecular weight: 51261.71 Da        Isoelectric Point: 4.7025

>T26238 WP_013578107.1 NC_017060:c424647-423298 [Rahnella aquatilis HX2]
MEFLTLQAFLNNTWVDIANITFPGSEQNNYRLTELNYHSDYAIDNLDRDDNHAASLNHPVSLFFDGDDERGWLKFLDDII
PSGSSRRYWANYLDLEGLTADQQNFILLKHGTLSPVGNLRIKESLPEYNPLAEKLFFTIEDVKNRAGDFLDYAQQRGAAA
GGATGAGGEAPKLLLRCSQEQQIWIDTYQNDTANRDIPYLVKYPRGARTEVDCNILRAEFYFYHELEAMGFDTIPTQNMR
LEEGLTYPSLWLPRFDISFEEEGLIRRFGMESVYSLLRKAPGVTLYHEETLRALIDKISHSHMVTEQGFDFDIPGFVTEW
VRRDLLNIIFGNSDNHGRNTSFLKDERSIRLAPIYDFAPMKADPEGIARTTKWKAPLEIGGEYDYPGIAQSLDDLVPAET
LLSALKNTSHQLSGLKERLEARGIPQQILNMPAISFSFIPEKLRRWGLL

Download         Length: 1350 bp

>T26238 NC_017060:c424647-423298 [Rahnella aquatilis HX2]
ATGGAATTTTTAACGTTACAAGCTTTCCTCAATAATACCTGGGTTGATATTGCTAACATCACTTTTCCCGGCAGTGAGCA
GAATAACTATCGTCTGACCGAACTGAATTATCATTCGGATTACGCTATTGATAACCTTGATCGTGACGATAACCATGCCG
CATCGTTGAATCACCCCGTCTCTTTGTTTTTTGATGGTGACGATGAGCGGGGTTGGCTGAAGTTTCTTGATGATATTATT
CCCAGCGGTTCAAGTCGCCGCTATTGGGCAAACTATCTCGATCTCGAAGGACTGACAGCCGATCAGCAAAATTTCATTTT
GCTCAAACATGGTACCTTGTCCCCTGTTGGCAATCTGCGCATAAAAGAATCATTACCGGAATATAATCCGCTGGCTGAAA
AACTTTTCTTCACTATAGAAGATGTTAAAAACCGGGCGGGTGACTTTCTGGACTACGCACAACAACGCGGTGCGGCTGCC
GGTGGCGCGACAGGCGCCGGGGGCGAAGCACCAAAGCTGTTGCTGCGTTGTAGTCAGGAACAGCAAATCTGGATTGATAC
CTACCAGAATGACACTGCCAACCGGGACATTCCCTATTTGGTCAAATATCCCCGTGGCGCAAGAACAGAGGTAGACTGCA
ATATTCTTCGGGCAGAATTCTATTTCTACCATGAGCTTGAAGCCATGGGGTTTGACACGATCCCGACGCAAAACATGCGG
CTGGAAGAAGGGCTGACTTATCCGTCACTCTGGCTTCCGCGTTTTGATATTTCTTTTGAAGAGGAAGGACTTATCAGACG
TTTCGGAATGGAATCAGTCTATTCACTCCTGCGCAAAGCGCCCGGTGTGACGCTGTATCATGAAGAAACCCTCCGTGCAC
TGATCGATAAGATTTCACACAGTCATATGGTGACAGAACAAGGTTTTGATTTTGATATCCCGGGGTTTGTGACTGAATGG
GTACGACGAGATCTGCTCAATATTATTTTTGGTAACAGCGATAATCATGGGCGTAACACCTCTTTCCTGAAAGATGAACG
CAGCATCCGGCTTGCACCAATCTACGATTTTGCGCCGATGAAAGCCGATCCGGAAGGTATTGCCAGAACGACGAAATGGA
AGGCTCCGCTGGAAATCGGCGGTGAATACGATTATCCCGGCATTGCACAATCACTCGATGATCTTGTCCCGGCAGAAACA
TTACTTTCGGCACTTAAAAACACGTCACATCAACTGTCGGGACTGAAAGAGCGGCTGGAAGCCCGTGGCATACCGCAGCA
AATTTTGAACATGCCCGCGATAAGTTTCAGTTTTATTCCTGAAAAGTTACGCCGCTGGGGGTTGTTATGA

Antitoxin


Download         Length: 158 a.a.        Molecular weight: 17587.18 Da        Isoelectric Point: 10.9653

>AT26238 WP_013578106.1 NC_017060:c423301-422828 [Rahnella aquatilis HX2]
MKEPEIAANSARNTIDKIRAKQNEANHVRRDAGQMLAQEETSSTGKKQAQRRVSNLTKPVSTNDRNQARLDIMRALLLGE
ITQGQALKALRITVLGLKQDAFGKLVTVSRKTLSEVENDKGNYTPDIINKLFRPFGLKVGLVPVSQQIFRTLFERSE

Download         Length: 474 bp

>AT26238 NC_017060:c423301-422828 [Rahnella aquatilis HX2]
ATGAAAGAACCAGAGATTGCCGCAAACTCTGCGAGAAATACCATTGATAAAATACGGGCTAAACAAAATGAGGCGAATCA
TGTGCGGCGTGACGCCGGACAAATGCTGGCTCAGGAAGAAACATCTTCCACGGGAAAAAAACAAGCCCAGCGAAGAGTGT
CGAACCTGACGAAACCCGTCAGCACCAATGACCGAAATCAAGCCCGGCTCGACATCATGCGGGCGTTACTTTTGGGTGAA
ATCACGCAGGGACAAGCACTAAAAGCACTGAGGATTACGGTGCTGGGGTTAAAACAAGACGCCTTTGGGAAACTAGTGAC
AGTGTCACGTAAGACGCTATCAGAAGTGGAAAATGATAAGGGAAATTACACGCCTGACATCATCAACAAACTGTTTAGAC
CATTTGGCCTGAAAGTCGGGCTGGTGCCGGTTTCTCAGCAGATATTCAGGACTCTCTTTGAGCGCAGTGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB H8P0A1

Antitoxin

Source ID Structure
AlphaFold DB H8P0A0

References