Detailed information of TA system TA026237

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 279827..281377
• Replicon: plasmid PRA1
• Accession: NC_017060
• Organism: Rahnella aquatilis HX2

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: H8P0L7
• Locus tag: Q7S_RS23820
• Protein ID: WP_013577980.1
• Coordinates: 279827..281122 (-)
• Length: 432 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: Q7S_RS23825
• Protein ID: WP_013577981.1
• Coordinates: 281126..281377 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Q7S_RS23810	276001..276888	+	888	WP_041673458.1	LysR family transcriptional regulator	-
Q7S_RS23815	277286..279763	+	2478	WP_041673396.1	membrane-bound PQQ-dependent dehydrogenase, glucose/quinate/shikimate family	-
Q7S_RS23820	279827..281122	-	1296	WP_013577980.1	type II toxin-antitoxin system HipA family toxin	Toxin
Q7S_RS23825	281126..281377	-	252	WP_013577981.1	helix-turn-helix domain-containing protein	Antitoxin
Q7S_RS23830	281585..282295	+	711	WP_014416611.1	GntR family transcriptional regulator	-
Q7S_RS23835	282306..283505	-	1200	WP_014416612.1	2-dehydro-3-deoxy-6-phosphogalactonate aldolase	-
Q7S_RS23840	283830..285230	+	1401	WP_014416613.1	PTS transporter subunit EIIC	-
Q7S_RS23845	285237..286109	-	873	WP_013577985.1	fumarylacetoacetate hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaRAHN-2	mgtB / vipA/tssB / vipB/tssC / hcp/tssD / clpV/tssH / hcp/tssD / hcp/tssD	1..570951	570951

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (6-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (10-64)

Sequences

Toxin

Protein

• Length: 432 a.a.
• Molecular weight: 48192.43 Da
• Isoelectric Point: 7.0668

>T26237 WP_013577980.1 NC_017060:c281122-279827 [Rahnella aquatilis HX2]
MDKLTLALNGIAVGTLEKGGSGEMSFVYHQSWLDRPGARAISLSLPLQADKFRGAVVYNFFDNLLPDNEQIRSRIQARFH
IPTKQPFDLLSRIGGDCVGAIQLYPEGDQVAPVTEIHAQPLGEKDIEQLLQGYKDAPLGMEADIEDFRISLAGAQEKTAL
LWYRDQWHRPQGSTPTSHILKLPIGYIANNGIDLSESVENEWLCLKIAEKFGFPVARSEIASFGAQKVLVVERFDRRWSQ
DGSWLMRLPQEDFCQALGVAPALKYESDGGPGIARAMKLLLGSQRAGADRDMFFKSQILFWMLAAIDGHAKNFSVYIEPG
SAYRMTPFYDVMSAFPLMHLQGIPAKKAKMAMAVLGKNRHFHWDRILPRHFVTTAQRVEYSKENVSAMMTEMKEKTEQVI
ADVEAIIPANFPAKISDAIFAGLRHQAARLP

Nucleotide

• Length: 1296 bp

>T26237 NC_017060:c281122-279827 [Rahnella aquatilis HX2]
ATGGATAAGTTAACGCTCGCATTAAATGGCATTGCTGTCGGTACCCTGGAAAAAGGCGGCAGTGGTGAAATGTCATTTGT
TTACCATCAAAGCTGGCTCGATCGCCCCGGAGCCCGCGCCATTTCATTATCATTACCTCTTCAGGCAGATAAATTCCGTG
GCGCAGTGGTATACAACTTTTTTGACAATCTTCTACCCGATAACGAACAGATACGCAGCCGTATTCAGGCACGTTTCCAC
ATCCCGACGAAGCAGCCTTTCGATTTGCTTTCGCGGATCGGTGGTGACTGTGTCGGTGCGATCCAGCTCTATCCTGAAGG
TGATCAGGTTGCACCTGTCACGGAAATTCATGCCCAACCGCTTGGCGAAAAGGATATTGAGCAGCTTCTGCAGGGATATA
AGGATGCACCTTTGGGCATGGAAGCTGACATTGAAGACTTTCGAATTTCTCTGGCCGGTGCACAGGAAAAAACGGCTTTG
CTCTGGTATCGCGACCAGTGGCATCGCCCGCAGGGAAGTACGCCTACCAGCCATATCCTTAAACTTCCTATTGGTTATAT
CGCTAACAATGGCATTGATCTCAGCGAGAGCGTGGAGAATGAATGGTTGTGTCTGAAGATAGCCGAAAAATTTGGATTTC
CTGTTGCACGGTCTGAGATTGCCAGTTTTGGCGCTCAGAAGGTGTTGGTCGTTGAGCGTTTTGACCGCCGCTGGTCGCAG
GATGGATCCTGGCTGATGCGTTTACCTCAGGAAGATTTTTGTCAGGCGCTGGGTGTTGCTCCCGCATTGAAGTATGAATC
TGATGGCGGCCCCGGAATTGCACGCGCAATGAAGCTTTTACTGGGTTCTCAACGGGCGGGGGCGGACAGGGATATGTTCT
TTAAAAGCCAGATCCTCTTCTGGATGCTGGCCGCCATTGACGGTCATGCTAAGAATTTCAGTGTTTATATCGAGCCGGGC
AGCGCATACCGCATGACACCGTTTTATGACGTGATGTCAGCATTTCCTCTGATGCATCTGCAAGGAATTCCGGCGAAGAA
AGCCAAAATGGCGATGGCTGTATTAGGGAAAAACCGGCATTTTCATTGGGACAGAATTCTTCCCCGCCATTTTGTGACGA
CGGCGCAACGGGTCGAGTACTCGAAAGAAAACGTCTCTGCGATGATGACGGAAATGAAGGAAAAAACTGAGCAGGTGATT
GCAGATGTTGAAGCAATAATACCTGCTAATTTTCCGGCAAAAATCAGTGATGCAATTTTTGCAGGCCTGCGCCATCAGGC
TGCACGTTTGCCTTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9570.88 Da
• Isoelectric Point: 7.9783

>AT26237 WP_013577981.1 NC_017060:c281377-281126 [Rahnella aquatilis HX2]
MNITSAKMLANALRNERKKRKLSQSKTAETIGLKQSTVSEFENYPDGTRLETLFKLLAALDLELQITPRDSNPQEKCTWD
QEW

Nucleotide

• Length: 252 bp

>AT26237 NC_017060:c281377-281126 [Rahnella aquatilis HX2]
ATGAACATTACATCTGCAAAAATGCTCGCCAATGCGCTGCGTAATGAGCGAAAAAAGCGCAAACTCAGCCAAAGTAAAAC
GGCTGAAACCATTGGCCTGAAGCAGTCTACGGTTTCTGAGTTCGAAAATTATCCGGACGGTACCCGTCTGGAAACACTGT
TCAAATTATTGGCGGCGCTGGATTTAGAGCTACAGATCACGCCAAGGGATAGCAATCCGCAGGAAAAGTGTACCTGGGAT
CAGGAGTGGTAA

Structures

Toxin

• AlphaFold DB: H8P0L7