262273

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 111394..112037 Replicon plasmid pEC488-1
Accession NZ_CP109875
Organism Escherichia coli strain EC488

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag NQ150_RS25735 Protein ID WP_001034044.1
Coordinates 111621..112037 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag NQ150_RS25730 Protein ID WP_001261286.1
Coordinates 111394..111624 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NQ150_RS25720 (106732) 106732..107157 - 426 WP_000422741.1 transposase -
NQ150_RS25725 (107228) 107228..111013 - 3786 WP_261630093.1 pcar -
NQ150_RS25730 (111394) 111394..111624 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
NQ150_RS25735 (111621) 111621..112037 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
NQ150_RS25740 (112112) 112112..113677 + 1566 WP_001128474.1 AAA family ATPase -
NQ150_RS25745 (113662) 113662..114684 + 1023 WP_000361402.1 helicase UvrD -
NQ150_RS25755 (115991) 115991..116905 + 915 WP_000949004.1 iron/manganese ABC transporter substrate-binding protein SitA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid dfrA17 / aadA5 / qacE / sul1 / mph(A) / tet(B) / sitABCD iucA / iucB / iucC / iucD / iutA 1..133748 133748
- flank IS/Tn sitABCD - 114938..119440 4502


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T262273 WP_001034044.1 NZ_CP109875:111621-112037 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT262273 WP_001261286.1 NZ_CP109875:111394-111624 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References