262272

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 103991..104634 Replicon plasmid pEC488-1
Accession NZ_CP109875
Organism Escherichia coli strain EC488

Toxin (Protein)


Gene name vagD Uniprot ID C7S9Y5
Locus tag NQ150_RS25705 Protein ID WP_001034046.1
Coordinates 104218..104634 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID V0SR71
Locus tag NQ150_RS25700 Protein ID WP_001261278.1
Coordinates 103991..104221 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NQ150_RS25670 (99502) 99502..99807 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB -
NQ150_RS25675 (99809) 99809..100027 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA -
NQ150_RS25680 (100618) 100618..101106 + 489 WP_011254646.1 hypothetical protein -
NQ150_RS25685 (101140) 101140..102273 - 1134 WP_001542067.1 DUF3800 domain-containing protein -
NQ150_RS25690 (102440) 102440..103213 - 774 WP_000905949.1 hypothetical protein -
NQ150_RS25695 (103226) 103226..103726 - 501 WP_000528931.1 HEPN family nuclease -
NQ150_RS25700 (103991) 103991..104221 + 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
NQ150_RS25705 (104218) 104218..104634 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin Toxin
NQ150_RS25710 (104741) 104741..106354 - 1614 WP_000080193.1 IS66-like element ISEc23 family transposase -
NQ150_RS25715 (106385) 106385..106735 - 351 WP_000624722.1 IS66 family insertion sequence element accessory protein TnpB -
NQ150_RS25720 (106732) 106732..107157 - 426 WP_000422741.1 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid dfrA17 / aadA5 / qacE / sul1 / mph(A) / tet(B) / sitABCD iucA / iucB / iucC / iucD / iutA 1..133748 133748


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-53)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14978.31 Da        Isoelectric Point: 6.7113

>T262272 WP_001034046.1 NZ_CP109875:104218-104634 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEVKVALRLAGTPIGPNDTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG

Download         Length: 417 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8853.07 Da        Isoelectric Point: 4.6437

>AT262272 WP_001261278.1 NZ_CP109875:103991-104221 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLRPSWSSFLELEKADPGFMTEREDVVCDEGRFNL

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9NXF9

Antitoxin

Source ID Structure
AlphaFold DB V0SR71

References