262251

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 3041221..3042005 Replicon chromosome
Accession NZ_CP109874
Organism Escherichia coli strain EC488

Toxin (Protein)


Gene name TacT2 Uniprot ID V0T0H9
Locus tag NQ150_RS14680 Protein ID WP_000613626.1
Coordinates 3041511..3042005 (+) Length 165 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID A0A0A1AEM7
Locus tag NQ150_RS14675 Protein ID WP_024946541.1
Coordinates 3041221..3041514 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NQ150_RS14665 (3036382) 3036382..3037341 - 960 WP_022645527.1 23S rRNA pseudouridine(955/2504/2580) synthase RluC -
NQ150_RS14670 (3037914) 3037914..3041087 + 3174 WP_022645526.1 ribonuclease E -
NQ150_RS14675 (3041221) 3041221..3041514 + 294 WP_024946541.1 DUF1778 domain-containing protein Antitoxin
NQ150_RS14680 (3041511) 3041511..3042005 + 495 WP_000613626.1 GNAT family N-acetyltransferase Toxin
NQ150_RS14685 (3042100) 3042100..3043053 - 954 WP_022645524.1 flagellar hook-associated protein FlgL -
NQ150_RS14690 (3043065) 3043065..3044708 - 1644 WP_022645523.1 flagellar hook-associated protein FlgK -
NQ150_RS14695 (3044774) 3044774..3045715 - 942 WP_001317765.1 flagellar assembly peptidoglycan hydrolase FlgJ -
NQ150_RS14700 (3045715) 3045715..3046812 - 1098 WP_022645522.1 flagellar basal body P-ring protein FlgI -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(81-144)

Antitoxin

Vibrio phage ICP1, Orf50

(14-88)


Sequences

Toxin        


Download         Length: 165 a.a.        Molecular weight: 18084.00 Da        Isoelectric Point: 8.2617

>T262251 WP_000613626.1 NZ_CP109874:3041511-3042005 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Download         Length: 495 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11039.60 Da        Isoelectric Point: 10.5423

>AT262251 WP_024946541.1 NZ_CP109874:3041221-3041514 [Escherichia coli]
MAAANNIPARRESLNLRIKPAERDLIDRAAKARGKNRSEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0T0H9

Antitoxin

Source ID Structure
AlphaFold DB A0A0A1AEM7

References