Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 248527..249169 Replicon chromosome
Accession NZ_CP109872
Organism Bacillus cereus strain BC38B

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag OK229_RS01405 Protein ID WP_000635963.1
Coordinates 248819..249169 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag OK229_RS01400 Protein ID WP_000004570.1
Coordinates 248527..248814 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OK229_RS01375 (OK229_11205) 243843..244805 + 963 WP_000961155.1 UV DNA damage repair endonuclease UvsE -
OK229_RS01380 (OK229_11210) 244798..245370 - 573 WP_000906926.1 rhomboid family intramembrane serine protease -
OK229_RS01385 (OK229_11215) 245463..245822 + 360 WP_000635040.1 holo-ACP synthase -
OK229_RS01390 (OK229_11220) 245979..246929 + 951 WP_025388382.1 outer membrane lipoprotein carrier protein LolA -
OK229_RS01395 (OK229_11225) 247048..248217 + 1170 WP_000390600.1 alanine racemase -
OK229_RS01400 (OK229_11230) 248527..248814 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
OK229_RS01405 (OK229_11235) 248819..249169 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
OK229_RS01410 (OK229_11240) 249237..251405 + 2169 WP_264459790.1 Tex family protein -
OK229_RS01415 (OK229_11245) 251464..251580 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
OK229_RS01420 (OK229_11250) 251776..252234 + 459 WP_073526447.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T262238 WP_000635963.1 NZ_CP109872:248819-249169 [Bacillus cereus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT262238 WP_000004570.1 NZ_CP109872:248527-248814 [Bacillus cereus]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References