262172

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Xre-MNT/HTH_26(antitoxin)
Location 75264..76372 Replicon plasmid pT17-1-1
Accession NZ_CP109841
Organism Enterococcus faecium strain T17-1

Toxin (Protein)


Gene name MNTss Uniprot ID R3JIN1
Locus tag OKL57_RS13565 Protein ID WP_000233000.1
Coordinates 75264..76133 (-) Length 290 a.a.

Antitoxin (Protein)


Gene name Xress Uniprot ID -
Locus tag OKL57_RS13570 Protein ID WP_000205227.1
Coordinates 76148..76372 (-) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OKL57_RS13535 (OKL57_13530) 70426..71106 + 681 WP_001015311.1 IS6-like element IS1216 family transposase -
OKL57_RS13540 (OKL57_13535) 71334..71906 - 573 WP_202074493.1 HTH domain-containing protein -
OKL57_RS13545 (OKL57_13540) 72182..72976 - 795 WP_001096887.1 aminoglycoside O-phosphotransferase APH(3')-IIIa -
OKL57_RS13550 (OKL57_13545) 73069..73611 - 543 WP_000627290.1 streptothricin N-acetyltransferase Sat4 -
OKL57_RS13555 (OKL57_13550) 73608..74516 - 909 WP_001255866.1 aminoglycoside nucleotidyltransferase ANT(6)-Ia -
OKL57_RS13560 (OKL57_13555) 74549..75283 - 735 WP_000662263.1 class I SAM-dependent methyltransferase -
OKL57_RS13565 (OKL57_13560) 75264..76133 - 870 WP_000233000.1 nucleotidyltransferase domain-containing protein Toxin
OKL57_RS13570 (OKL57_13565) 76148..76372 - 225 WP_000205227.1 helix-turn-helix transcriptional regulator Antitoxin
OKL57_RS13575 (OKL57_13570) 76515..76744 - 230 Protein_90 hypothetical protein -
OKL57_RS13580 (OKL57_13575) 76810..78092 + 1283 Protein_91 ISL3 family transposase -
OKL57_RS13585 (OKL57_13580) 78401..79204 - 804 WP_002294514.1 lincosamide nucleotidyltransferase Lnu(B) -
OKL57_RS13590 (OKL57_13585) 79258..80742 - 1485 WP_002294513.1 ABC-F type ribosomal protection protein Lsa(E) -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Polymerase, nucleotidyl transferase domain

(12-111)

Antitoxin

Cro/C1-type helix-turn-helix domain

(4-57)


Sequences

Toxin        


Download         Length: 290 a.a.        Molecular weight: 32926.57 Da        Isoelectric Point: 4.8781

>T262172 WP_000233000.1 NZ_CP109841:c76133-75264 [Enterococcus faecium]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Download         Length: 870 bp

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8365.68 Da        Isoelectric Point: 4.5510

>AT262172 WP_000205227.1 NZ_CP109841:c76372-76148 [Enterococcus faecium]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Download         Length: 225 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB R3JIN1

Antitoxin

Source ID Structure

References