262171

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 8255..9392 Replicon plasmid pT17-1-optrA-57k
Accession NZ_CP109840
Organism Enterococcus faecium strain T17-1

Toxin (Protein)


Gene name zeta Uniprot ID Q54944
Locus tag OKL57_RS12835 Protein ID WP_001284311.1
Coordinates 8255..9118 (-) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag OKL57_RS12840 Protein ID WP_000301765.1
Coordinates 9120..9392 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OKL57_RS12800 (OKL57_12795) 4482..4625 - 144 WP_002360221.1 hypothetical protein -
OKL57_RS12805 (OKL57_12800) 5073..5207 + 135 Protein_4 DNA repair protein RadC -
OKL57_RS12810 (OKL57_12805) 5430..5813 + 384 Protein_5 N-6 DNA methylase -
OKL57_RS12815 (OKL57_12810) 5869..6549 - 681 WP_001015311.1 IS6-like element IS1216 family transposase -
OKL57_RS12820 (OKL57_12815) 6603..6725 - 123 WP_159111623.1 DpnD/PcfM family protein -
OKL57_RS12825 (OKL57_12820) 6760..7257 - 498 WP_010713926.1 molecular chaperone DnaJ -
OKL57_RS12830 (OKL57_12825) 7791..8108 - 318 WP_002300567.1 hypothetical protein -
OKL57_RS12835 (OKL57_12830) 8255..9118 - 864 WP_001284311.1 zeta toxin family protein Toxin
OKL57_RS12840 (OKL57_12835) 9120..9392 - 273 WP_000301765.1 antitoxin Antitoxin
OKL57_RS12845 (OKL57_12840) 9409..9624 - 216 WP_001835296.1 peptide-binding protein -
OKL57_RS12850 (OKL57_12845) 9758..10018 - 261 Protein_13 topoisomerase DNA-binding C4 zinc finger domain-containing protein -
OKL57_RS12855 (OKL57_12850) 10188..10931 - 744 Protein_14 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
OKL57_RS12860 (OKL57_12855) 11050..11133 - 84 WP_001865637.1 23S rRNA methyltransferase attenuation leader peptide -
OKL57_RS12865 (OKL57_12860) 11182..11421 - 240 WP_000635249.1 peptide-binding protein -
OKL57_RS12870 (OKL57_12865) 11555..13699 - 2145 WP_264460529.1 type IA DNA topoisomerase -
OKL57_RS12875 (OKL57_12870) 13699..14316 - 618 WP_001062589.1 recombinase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid optrA / fexA / erm(B) / erm(A) - 1..57547 57547
- inside IS/Tn optrA / fexA / erm(B) - 518..10925 10407


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32404.02 Da        Isoelectric Point: 6.9964

>T262171 WP_001284311.1 NZ_CP109840:c9118-8255 [Enterococcus faecium]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLEKELNRKVSGKEIQ
PTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT262171 WP_000301765.1 NZ_CP109840:c9392-9120 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1GVN
PDB 3Q8X

Antitoxin

Source ID Structure
AlphaFold DB A0A829F0A3

References