262124

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Xre-MNT/HTH_26(antitoxin)
Location 93880..94988 Replicon plasmid pTF51-2-1
Accession NZ_CP109800
Organism Enterococcus faecium strain TF51-2

Toxin (Protein)


Gene name MNTss Uniprot ID R3JIN1
Locus tag OKL55_RS13660 Protein ID WP_000233000.1
Coordinates 93880..94749 (-) Length 290 a.a.

Antitoxin (Protein)


Gene name Xress Uniprot ID -
Locus tag OKL55_RS13665 Protein ID WP_000205227.1
Coordinates 94764..94988 (-) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OKL55_RS13630 (OKL55_13635) 89299..90036 - 738 WP_002321849.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
OKL55_RS13635 (OKL55_13640) 90161..90256 - 96 WP_001809736.1 23S rRNA methyltransferase attenuation leader peptide -
OKL55_RS13640 (OKL55_13645) 90798..91592 - 795 WP_001096887.1 aminoglycoside O-phosphotransferase APH(3')-IIIa -
OKL55_RS13645 (OKL55_13650) 91685..92227 - 543 WP_000627290.1 streptothricin N-acetyltransferase Sat4 -
OKL55_RS13650 (OKL55_13655) 92224..93132 - 909 WP_001255866.1 aminoglycoside nucleotidyltransferase ANT(6)-Ia -
OKL55_RS13655 (OKL55_13660) 93165..93899 - 735 WP_000662263.1 class I SAM-dependent methyltransferase -
OKL55_RS13660 (OKL55_13665) 93880..94749 - 870 WP_000233000.1 nucleotidyltransferase domain-containing protein Toxin
OKL55_RS13665 (OKL55_13670) 94764..94988 - 225 WP_000205227.1 helix-turn-helix transcriptional regulator Antitoxin
OKL55_RS13670 (OKL55_13675) 95131..95265 - 135 Protein_106 site-specific recombinase -
OKL55_RS13675 (OKL55_13680) 95406..96707 + 1302 Protein_107 ISL3 family transposase -
OKL55_RS13680 (OKL55_13685) 97016..97819 - 804 WP_002294514.1 lincosamide nucleotidyltransferase Lnu(B) -
OKL55_RS13685 (OKL55_13690) 97873..99357 - 1485 WP_002294513.1 ABC-F type ribosomal protection protein Lsa(E) -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Polymerase, nucleotidyl transferase domain

(12-111)

Antitoxin

Cro/C1-type helix-turn-helix domain

(4-57)


Sequences

Toxin        


Download         Length: 290 a.a.        Molecular weight: 32926.57 Da        Isoelectric Point: 4.8781

>T262124 WP_000233000.1 NZ_CP109800:c94749-93880 [Enterococcus faecium]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Download         Length: 870 bp

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8365.68 Da        Isoelectric Point: 4.5510

>AT262124 WP_000205227.1 NZ_CP109800:c94988-94764 [Enterococcus faecium]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Download         Length: 225 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB R3JIN1

Antitoxin

Source ID Structure

References