262114

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 533616..534253 Replicon chromosome
Accession NZ_CP109795
Organism Bacillus velezensis strain B19

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag L0P93_RS02845 Protein ID WP_003156187.1
Coordinates 533903..534253 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag L0P93_RS02840 Protein ID WP_003156188.1
Coordinates 533616..533897 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L0P93_RS02820 (529982) 529982..530581 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
L0P93_RS02825 (530674) 530674..531039 + 366 WP_014304402.1 holo-ACP synthase -
L0P93_RS02830 (531194) 531194..532210 + 1017 WP_104679029.1 outer membrane lipoprotein carrier protein LolA -
L0P93_RS02835 (532327) 532327..533496 + 1170 WP_104679030.1 alanine racemase -
L0P93_RS02840 (533616) 533616..533897 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
L0P93_RS02845 (533903) 533903..534253 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
L0P93_RS02850 (534371) 534371..535192 + 822 WP_003156182.1 STAS domain-containing protein -
L0P93_RS02855 (535197) 535197..535562 + 366 WP_007609584.1 RsbT antagonist protein RsbS -
L0P93_RS02860 (535565) 535565..535966 + 402 WP_003156178.1 anti-sigma regulatory factor -
L0P93_RS02865 (535978) 535978..536985 + 1008 WP_007609589.1 PP2C family protein-serine/threonine phosphatase -
L0P93_RS02870 (537049) 537049..537378 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
L0P93_RS02875 (537375) 537375..537857 + 483 WP_007609591.1 anti-sigma B factor RsbW -
L0P93_RS02880 (537823) 537823..538611 + 789 WP_029974100.1 RNA polymerase sigma factor SigB -
L0P93_RS02885 (538611) 538611..539213 + 603 WP_007410234.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T262114 WP_003156187.1 NZ_CP109795:533903-534253 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT262114 WP_003156188.1 NZ_CP109795:533616-533897 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References