Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 202625..203250 Replicon plasmid pINV
Accession NZ_CP109776
Organism Shigella sonnei strain CIP_106347

Toxin (Protein)


Gene name vapC Uniprot ID Q6XVU6
Locus tag OHN14_RS25500 Protein ID WP_000911318.1
Coordinates 202625..203023 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q31SG2
Locus tag OHN14_RS25505 Protein ID WP_000450530.1
Coordinates 203023..203250 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OHN14_RS25480 (OHN14_25480) 198637..198975 + 339 Protein_235 antirestriction protein -
OHN14_RS25485 (OHN14_25485) 199041..200197 + 1157 WP_087879933.1 IS3-like element IS600 family transposase -
OHN14_RS25490 (OHN14_25490) 200240..200371 + 132 Protein_237 IS1 family transposase -
OHN14_RS25495 (OHN14_25495) 200397..202616 + 2220 WP_077141270.1 type IV conjugative transfer system coupling protein TraD -
OHN14_RS25500 (OHN14_25500) 202625..203023 - 399 WP_000911318.1 type II toxin-antitoxin system VapC family toxin Toxin
OHN14_RS25505 (OHN14_25505) 203023..203250 - 228 WP_000450530.1 toxin-antitoxin system antitoxin VapB Antitoxin
OHN14_RS25510 (OHN14_25510) 203332..207603 + 4272 Protein_241 conjugative transfer relaxase/helicase TraI -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ipaH1.4 / tviC / nleE / icsP/sopA / ospB / ospC4 / ospD2 / ospF / ospD1 / ipgB2 / ospE2 / ospC1 / ospD3/senA / ipaH7.8 / ipaH4.5 / ospC2 / ospC3 / ipaJ / ipaA / ipaD / ipaC / ipaB / ipgC / ipgB1 / ipgA / icsB / ipgD / ipgE / ipgF / mxiG / mxiH / mxiI / mxiJ / mxiK / mxiN / mxiL / mxiM / mxiE / mxiD / mxiC / mxiA / spa15 / spa47 / spa13 / spa32 / spa33 / spa24 / spa9 / spa29 / spa40 / virA / icsA/virG / papC / ospI / ipaH9.8 / ospG 1..214749 214749


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14845.09 Da        Isoelectric Point: 8.5264

>T262112 WP_000911318.1 NZ_CP109776:c203023-202625 [Shigella sonnei]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVGGLRTEDWS

Download         Length: 399 bp

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8535.59 Da        Isoelectric Point: 4.7513

>AT262112 WP_000450530.1 NZ_CP109776:c203250-203023 [Shigella sonnei]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHSVSADFMDNREQPGMQERESF

Download         Length: 228 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6SD6

Antitoxin

Source ID Structure
AlphaFold DB Q31SG2

References