Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 103313..104936 Replicon plasmid p2017-45-51-04-01_1
Accession NZ_CP109730
Organism Enterobacter roggenkampii strain 2017-45-51-04-01

Toxin (Protein)


Gene name hipA Uniprot ID C1IUI3
Locus tag OI980_RS25805 Protein ID WP_000088044.1
Coordinates 103313..104638 (-) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A6M3PBR7
Locus tag OI980_RS25810 Protein ID WP_012477377.1
Coordinates 104643..104936 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OI980_RS25780 (OI980_25770) 100287..100721 + 435 WP_000405672.1 Cd(II)/Pb(II)-responsive transcriptional regulator -
OI980_RS25785 (OI980_25775) 100812..101816 + 1005 WP_000427623.1 IS110-like element IS4321 family transposase -
OI980_RS25790 (OI980_25780) 101998..102480 - 483 WP_001139512.1 hypothetical protein -
OI980_RS25795 (OI980_25785) 102535..102945 - 411 WP_000985909.1 hypothetical protein -
OI980_RS25800 (OI980_25790) 103046..103252 - 207 WP_000134171.1 hypothetical protein -
OI980_RS25805 (OI980_25795) 103313..104638 - 1326 WP_000088044.1 type II toxin-antitoxin system HipA family toxin Toxin
OI980_RS25810 (OI980_25800) 104643..104936 - 294 WP_012477377.1 helix-turn-helix transcriptional regulator Antitoxin
OI980_RS25815 (OI980_25805) 105337..106725 - 1389 WP_012695445.1 hypothetical protein -
OI980_RS25820 (OI980_25810) 106730..107227 - 498 WP_000340139.1 membrane protein -
OI980_RS25825 (OI980_25815) 107430..108086 - 657 WP_000462754.1 trypsin-like serine protease -
OI980_RS25830 (OI980_25820) 108745..109137 - 393 WP_000341066.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(D) / blaTEM-1B / sul1 / qacE / ere(A) / aac(6')-IIc / mcr-9 - 1..256678 256678
- flank IS/Tn - - 100812..101816 1004


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(8-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50333.32 Da        Isoelectric Point: 6.5044

>T262027 WP_000088044.1 NZ_CP109730:c104638-103313 [Enterobacter roggenkampii]
MSRKQQRLAIWMNGIKVGYWEKSKGVDSLQYLPDWSADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMRSSVENEWLCSVLLEQYGLPVARTQIAHFEDQKALVVERF
DRKWSGNGQWIIRLPQEDMCQALGISPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIVFWMMAATDGHAKNFS
ISIGPQGRYHLTPLYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10860.30 Da        Isoelectric Point: 10.3134

>AT262027 WP_012477377.1 NZ_CP109730:c104936-104643 [Enterobacter roggenkampii]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSTTASSEGK
QTEEMLKSNSPARQEKW

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2S1XXW1

Antitoxin

Source ID Structure
AlphaFold DB A0A6M3PBR7

References