Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazF-pemI/MazF(toxin)
Location 3438504..3439137 Replicon chromosome
Accession NZ_CP109616
Organism Weizmannia sp. WK01

Toxin (Protein)


Gene name mazF Uniprot ID G2TIM0
Locus tag OF158_RS16375 Protein ID WP_013860680.1
Coordinates 3438504..3438854 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID G2TIL9
Locus tag OF158_RS16380 Protein ID WP_014096808.1
Coordinates 3438859..3439137 (-) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OF158_RS16340 (OF158_16340) 3433761..3434540 - 780 WP_014096815.1 RNA polymerase sigma factor SigB -
OF158_RS16345 (OF158_16345) 3434518..3434988 - 471 WP_014096814.1 anti-sigma B factor RsbW -
OF158_RS16350 (OF158_16350) 3434992..3435327 - 336 WP_026104587.1 anti-sigma factor antagonist -
OF158_RS16355 (OF158_16355) 3435397..3436407 - 1011 WP_017551130.1 PP2C family protein-serine/threonine phosphatase -
OF158_RS16360 (OF158_16360) 3436421..3436822 - 402 WP_014096811.1 anti-sigma regulatory factor -
OF158_RS16365 (OF158_16365) 3436827..3437183 - 357 WP_014096810.1 STAS domain-containing protein -
OF158_RS16370 (OF158_16370) 3437187..3438014 - 828 WP_014096809.1 RsbT co-antagonist protein RsbRA -
OF158_RS16375 (OF158_16375) 3438504..3438854 - 351 WP_013860680.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
OF158_RS16380 (OF158_16380) 3438859..3439137 - 279 WP_014096808.1 hypothetical protein Antitoxin
OF158_RS16385 (OF158_16385) 3439290..3440441 - 1152 WP_014096807.1 alanine racemase -
OF158_RS16390 (OF158_16390) 3440643..3441659 - 1017 WP_035183268.1 outer membrane lipoprotein carrier protein LolA -
OF158_RS16395 (OF158_16395) 3441800..3442150 - 351 WP_014096805.1 holo-ACP synthase -
OF158_RS16400 (OF158_16400) 3442431..3443030 + 600 WP_014096804.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12968.01 Da        Isoelectric Point: 4.8998

>T261960 WP_013860680.1 NZ_CP109616:c3438854-3438504 [Weizmannia sp. WK01]
MIVKRGDVYFADLSPVVGSEQGGVRPVLILQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGLERDSVILLEQI
RTIDKQRLTDKITHLDEEVMEKIDDALQISLGLVEF

Download         Length: 351 bp

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 10633.19 Da        Isoelectric Point: 5.2204

>AT261960 WP_014096808.1 NZ_CP109616:c3439137-3438859 [Weizmannia sp. WK01]
VSEPISTEILVRLPQQLLTELDGFAETENMNRNELIYRATKMYLKERKKRQIRETMRRGYMEMAKINLAIASEAIQAEYE
AEHTVESLVSGG

Download         Length: 279 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B4BYJ0

Antitoxin

Source ID Structure
AlphaFold DB A0A8B4BX66

References