Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5153101..5153726 Replicon chromosome
Accession NZ_CP109614
Organism Klebsiella sp. KP20-425-1

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag OHJ17_RS25100 Protein ID WP_002882817.1
Coordinates 5153101..5153484 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag OHJ17_RS25105 Protein ID WP_004150355.1
Coordinates 5153484..5153726 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OHJ17_RS25085 (5150467) 5150467..5151369 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
OHJ17_RS25090 (5151366) 5151366..5152001 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
OHJ17_RS25095 (5151998) 5151998..5152927 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
OHJ17_RS25100 (5153101) 5153101..5153484 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
OHJ17_RS25105 (5153484) 5153484..5153726 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
OHJ17_RS25110 (5153931) 5153931..5154848 + 918 WP_021466676.1 alpha/beta hydrolase -
OHJ17_RS25115 (5154862) 5154862..5155803 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
OHJ17_RS25120 (5155848) 5155848..5156285 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
OHJ17_RS25125 (5156282) 5156282..5157142 - 861 WP_004146232.1 virulence factor BrkB family protein -
OHJ17_RS25130 (5157136) 5157136..5157735 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T261958 WP_002882817.1 NZ_CP109614:c5153484-5153101 [Klebsiella sp. KP20-425-1]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT261958 WP_004150355.1 NZ_CP109614:c5153726-5153484 [Klebsiella sp. KP20-425-1]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References