Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4559191..4560001 Replicon chromosome
Accession NZ_CP109614
Organism Klebsiella sp. KP20-425-1

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag OHJ17_RS22305 Protein ID WP_002887280.1
Coordinates 4559191..4559724 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag OHJ17_RS22310 Protein ID WP_002887278.1
Coordinates 4559735..4560001 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OHJ17_RS22300 (4558023) 4558023..4559144 + 1122 WP_009309849.1 cupin domain-containing protein -
OHJ17_RS22305 (4559191) 4559191..4559724 - 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
OHJ17_RS22310 (4559735) 4559735..4560001 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
OHJ17_RS22315 (4560104) 4560104..4561537 - 1434 WP_023288193.1 Cu(+)/Ag(+) sensor histidine kinase -
OHJ17_RS22320 (4561527) 4561527..4562210 - 684 WP_032105399.1 copper response regulator transcription factor CusR -
OHJ17_RS22325 (4562383) 4562383..4563768 + 1386 WP_032431458.1 efflux transporter outer membrane subunit -
OHJ17_RS22330 (4563786) 4563786..4564091 + 306 WP_032432083.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T261955 WP_002887280.1 NZ_CP109614:c4559724-4559191 [Klebsiella sp. KP20-425-1]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT261955 WP_002887278.1 NZ_CP109614:c4560001-4559735 [Klebsiella sp. KP20-425-1]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References