Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 1466726..1467462 Replicon chromosome
Accession NZ_CP109614
Organism Klebsiella sp. KP20-425-1

Toxin (Protein)


Gene name tacT Uniprot ID A0A4S4Y1H2
Locus tag OHJ17_RS07260 Protein ID WP_032433360.1
Coordinates 1466980..1467462 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag OHJ17_RS07255 Protein ID WP_003026799.1
Coordinates 1466726..1466992 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OHJ17_RS07230 (1462372) 1462372..1463511 + 1140 WP_032433368.1 mannitol dehydrogenase -
OHJ17_RS07235 (1463540) 1463540..1464202 + 663 WP_032433366.1 dihydroxyacetone kinase subunit DhaL -
OHJ17_RS07240 (1464186) 1464186..1465190 + 1005 WP_032433364.1 dihydroxyacetone kinase subunit DhaK -
OHJ17_RS07245 (1465208) 1465208..1465840 + 633 WP_032433362.1 3-hexulose-6-phosphate synthase -
OHJ17_RS07250 (1465850) 1465850..1466413 + 564 WP_032433361.1 6-phospho-3-hexuloisomerase -
OHJ17_RS07255 (1466726) 1466726..1466992 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
OHJ17_RS07260 (1466980) 1466980..1467462 + 483 WP_032433360.1 GNAT family N-acetyltransferase Toxin
OHJ17_RS07265 (1467662) 1467662..1469065 + 1404 WP_267303048.1 ISNCY-like element ISKpn21 family transposase -
OHJ17_RS07270 (1469094) 1469094..1469325 - 232 Protein_1419 hypothetical protein -
OHJ17_RS07275 (1469588) 1469588..1470187 - 600 WP_032432064.1 helix-turn-helix transcriptional regulator -
OHJ17_RS07280 (1470400) 1470400..1471344 - 945 WP_077254249.1 fimbrial protein -
OHJ17_RS07285 (1471356) 1471356..1471934 - 579 WP_032432061.1 type 1 fimbrial protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1434671..1480170 45499
- flank IS/Tn - - 1467662..1469065 1403


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(72-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17421.02 Da        Isoelectric Point: 9.6047

>T261948 WP_032433360.1 NZ_CP109614:1466980-1467462 [Klebsiella sp. KP20-425-1]
VGRVTAPEPLSSSHQLAEFVSGETVLDEWLKHRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTETTGRLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYTHHGFTASQTHERTLFLKLP

Download         Length: 483 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT261948 WP_003026799.1 NZ_CP109614:1466726-1466992 [Klebsiella sp. KP20-425-1]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4S4Y1H2

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References