Detailed information of TA system TA026194

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-yefM/Txe-RelB
• Location: 201647..202154
• Replicon: chromosome
• Accession: NC_017042
• Organism: Rickettsia rhipicephali str. 3-7-female6-CWPP

Toxin (Protein)

• Gene name: relB
• Uniprot ID: A0A0S2CBQ5
• Locus tag: MCC_RS01135
• Protein ID: WP_014408181.1
• Coordinates: 201647..201910 (-)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: H6QKN5
• Locus tag: MCC_RS01140
• Protein ID: WP_012152411.1
• Coordinates: 201903..202154 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MCC_RS08775	196726..196897	+	172	Protein_221	palindromic element RPE4 domain-containing protein	-
MCC_RS01105	197143..197549	+	407	Protein_222	hypothetical protein	-
MCC_RS07170	197663..198079	+	417	WP_196792984.1	Sca4 family protein	-
MCC_RS01120	198183..199577	-	1395	WP_014408178.1	lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase	-
MCC_RS01125	199574..200230	-	657	WP_014408179.1	lysophospholipid acyltransferase family protein	-
MCC_RS01130	200382..201587	-	1206	WP_014408180.1	pyridoxal phosphate-dependent aminotransferase	-
MCC_RS01135	201647..201910	-	264	WP_014408181.1	Txe/YoeB family addiction module toxin	Toxin
MCC_RS01140	201903..202154	-	252	WP_012152411.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
MCC_RS01145	202296..203300	+	1005	WP_014408182.1	methyltransferase domain-containing protein	-
MCC_RS01150	203305..204060	+	756	WP_041405474.1	VacJ family lipoprotein	-
MCC_RS01155	204080..204679	+	600	WP_014408184.1	phospholipid-binding protein MlaC	-
MCC_RS01160	204867..205061	+	195	WP_041405394.1	ribose-phosphate pyrophosphokinase-like domain-containing protein	-
MCC_RS01165	205061..205588	+	528	WP_196792986.1	ribose-phosphate diphosphokinase	-
MCC_RS01170	205597..205815	+	219	WP_014408185.1	palindromic element RPE1 domain-containing protein	-
MCC_RS01175	205885..206952	+	1068	WP_014408186.1	alanine racemase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (6-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10540.28 Da
• Isoelectric Point: 9.6105

>T26194 WP_014408181.1 NC_017042:c201910-201647 [Rickettsia rhipicephali str. 3-7-female6-CWPP]
MIEIEWTLEAAEDLAYWKKYDIKKYERIKLLIKNIQEAPFTGIGKPESLKDILSGLWSRRINHEHRLIYSVNTKQIIIYN
CRFHYKN

Nucleotide

• Length: 264 bp

>T26194 NC_017042:c201910-201647 [Rickettsia rhipicephali str. 3-7-female6-CWPP]
ATGATTGAGATTGAGTGGACACTAGAAGCAGCGGAGGATTTAGCGTATTGGAAAAAATACGATATTAAAAAATATGAACG
TATTAAACTCCTAATAAAAAATATCCAAGAAGCACCGTTTACAGGTATAGGTAAGCCCGAATCTTTAAAAGATATATTAT
CAGGTTTATGGTCACGTAGAATTAACCACGAACATAGACTAATATATTCTGTCAATACTAAACAAATTATAATATATAAT
TGTAGATTTCATTATAAAAATTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9544.81 Da
• Isoelectric Point: 9.4889

>AT26194 WP_012152411.1 NC_017042:c202154-201903 [Rickettsia rhipicephali str. 3-7-female6-CWPP]
MHSISGTSFRKNLSSVLNTVENDHVPYLIKRKNHKNIILLTEEEYESTKETLYLLSNPANANRIKKSIEQAKRGEFAKVN
LDD

Nucleotide

• Length: 252 bp

>AT26194 NC_017042:c202154-201903 [Rickettsia rhipicephali str. 3-7-female6-CWPP]
ATGCACTCAATTAGCGGCACTTCATTTAGAAAAAACTTAAGCTCTGTACTAAATACCGTAGAAAACGATCATGTCCCCTA
TCTTATTAAAAGAAAGAATCATAAGAATATTATTCTTTTAACCGAAGAAGAATATGAATCTACAAAAGAAACATTATATT
TATTATCTAATCCGGCTAATGCGAATCGAATAAAAAAATCTATCGAACAAGCAAAAAGAGGAGAGTTTGCTAAAGTTAAT
TTAGATGATTGA

Structures

Toxin

• AlphaFold DB: A0A0S2CBQ5

Antitoxin

• AlphaFold DB: H6QKN5