Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5249609..5250234 Replicon chromosome
Accession NZ_CP109607
Organism Klebsiella pneumoniae strain KP18-231

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag OGW23_RS25775 Protein ID WP_002882817.1
Coordinates 5249609..5249992 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag OGW23_RS25780 Protein ID WP_004150355.1
Coordinates 5249992..5250234 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OGW23_RS25760 (OGW23_25765) 5246975..5247877 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
OGW23_RS25765 (OGW23_25770) 5247874..5248509 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
OGW23_RS25770 (OGW23_25775) 5248506..5249435 + 930 WP_193525095.1 formate dehydrogenase accessory protein FdhE -
OGW23_RS25775 (OGW23_25780) 5249609..5249992 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
OGW23_RS25780 (OGW23_25785) 5249992..5250234 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
OGW23_RS25785 (OGW23_25790) 5250439..5251356 + 918 WP_002882812.1 alpha/beta hydrolase -
OGW23_RS25790 (OGW23_25795) 5251370..5252311 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
OGW23_RS25795 (OGW23_25800) 5252356..5252793 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
OGW23_RS25800 (OGW23_25805) 5252790..5253650 - 861 WP_002882807.1 virulence factor BrkB family protein -
OGW23_RS25805 (OGW23_25810) 5253644..5254243 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T261917 WP_002882817.1 NZ_CP109607:c5249992-5249609 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT261917 WP_004150355.1 NZ_CP109607:c5250234-5249992 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References