Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4529845..4530655 Replicon chromosome
Accession NZ_CP109604
Organism Klebsiella pneumoniae strain KP2022

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag KP2022_RS22105 Protein ID WP_004178461.1
Coordinates 4529845..4530378 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag KP2022_RS22110 Protein ID WP_002887278.1
Coordinates 4530389..4530655 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KP2022_RS22100 (4528676) 4528676..4529797 + 1122 WP_101971391.1 cupin domain-containing protein -
KP2022_RS22105 (4529845) 4529845..4530378 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
KP2022_RS22110 (4530389) 4530389..4530655 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
KP2022_RS22115 (4530758) 4530758..4532191 - 1434 WP_129073943.1 Cu(+)/Ag(+) sensor histidine kinase -
KP2022_RS22120 (4532181) 4532181..4532864 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
KP2022_RS22125 (4533037) 4533037..4534422 + 1386 WP_029602902.1 efflux transporter outer membrane subunit -
KP2022_RS22130 (4534440) 4534440..4534745 + 306 WP_032432083.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T261894 WP_004178461.1 NZ_CP109604:c4530378-4529845 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT261894 WP_002887278.1 NZ_CP109604:c4530655-4530389 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References