Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4029553..4030129 Replicon chromosome
Accession NZ_CP109601
Organism Escherichia coli strain TL-14

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag OGV15_RS19635 Protein ID WP_001297643.1
Coordinates 4029842..4030129 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag OGV15_RS19630 Protein ID WP_000063156.1
Coordinates 4029553..4029855 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OGV15_RS19615 (4026149) 4026149..4028299 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
OGV15_RS19620 (4028349) 4028349..4028552 + 204 WP_000467859.1 YbdD/YjiX family protein -
OGV15_RS19625 (4028563) 4028563..4029519 + 957 WP_001297640.1 GTPase -
OGV15_RS19630 (4029553) 4029553..4029855 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
OGV15_RS19635 (4029842) 4029842..4030129 - 288 WP_001297643.1 BrnT family toxin Toxin
OGV15_RS19640 (4030328) 4030328..4031025 - 698 Protein_3838 IS1 family transposase -
OGV15_RS19645 (4031082) 4031082..4031177 + 96 Protein_3839 dienelactone hydrolase family protein -
OGV15_RS19650 (4031323) 4031323..4032555 + 1233 WP_001380906.1 multidrug efflux MFS transporter MdtM -
OGV15_RS19655 (4032596) 4032596..4033876 + 1281 WP_001037380.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4030328..4030537 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T261864 WP_001297643.1 NZ_CP109601:c4030129-4029842 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT261864 WP_000063156.1 NZ_CP109601:c4029855-4029553 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References